牙科植體用鈦表面製作含銀氫氧基磷灰石鍍層之研究

純鈦在牙科植體領域被廣泛的應用，然而如果植體周圍有細菌感染問題將造成發炎現象，進而導致植體失敗。氫氧基磷灰石(HA)由於廣泛被用作骨重建的生物材料，因此也常被作為鈦植體表面改質材料，藉以改善鈦植體的骨傳導性及生物活性。銀因具有抑制細菌生長的抗菌性，因此本研究試圖在純鈦表面製備含銀氫氧基磷灰石(Ag-HA)鍍層，藉以增加鈦植體的抗菌性及生物活性。研究使用商業用純鈦(c.p. Ti)作為基材，所有樣品均用砂紙研磨，之後放置於超音波震盪機中分別用乙醇及去離子水進行洗淨，將蛋殼溶解後，在蛋殼溶液中加入AgNO3 作為沉積的電解液。在不同時間下對HA 進行電沉積處理。沉積時間分別為3 分鐘及5 分鐘。結果顯示純鈦表面沉積的鍍層主要為氫氧基磷灰石結構。HA 及Ag-HA 的鍍層顯現出為針狀結晶。EDS 分析證明Ag-HA 鍍層中有銀的成分。接觸角分析結果發現HA 及Ag-HA 鍍層皆具有低的水滴接觸角，HA 鍍層及Ag-HA 鍍層之水滴接觸角分別為17.3˚及26.7˚，表示鍍層具有好的親水性。鍍層與純鈦的結合強度結果顯示HA 鍍層為27.4 MPa，Ag-HA 鍍層為28.8 MPa，均達到25 MPa 以上。生物相容性結果顯示Ag-HA 鍍層細胞活性及貼附情形顯示出較佳的生物相容性。抗菌試驗結果顯示Ag-HA 鍍層上細菌之OD 值為0.16， HA 鍍層OD 值為0.63，說明添加銀確實具有良好的抗菌性。

關鍵字

抗菌性；銀；氫氧基磷灰石；純鈦

並列摘要

Pure titanium is widely used in dental implants. However, if there is a bacterial infection around the implant, it will cause inflammation and the implant to fail. Hydroxyapatite (HA) is widely used for bone reconstruction, and it is often used as a surface modification material to improve the osteoconductivity and biological activity of titanium implants. Silver has the antibacterial property of inhibiting the growth of bacteria. The aim of this study tried to prepare silver-containing hydroxyapatite (Ag-HA) coating on the surface of pure titanium to increase the antibacterial and biological activity of titanium implants. The research uses commercial pure titanium (c.p. Ti) as the substrate. All samples are ground with sandpaper and then placed in an ultrasonic vibrator to wash with ethanol and deionized water. The eggshell is dissolved in the eggshell solution and added AgNO3 as the electrolyte for deposition. The electrodeposition of HA was carried out at different times 3 minutes and 5 minutes, respectively. The results show that the coating deposited on the surface of pure titanium is mainly a hydroxide apatite structure. The coatings of HA and Ag-HA appear to be needle-like crystals. EDS analysis proved that there is silver in the Ag-HA coating. The results of the contact angle analysis found that both HA and Ag-HA coatings have low water droplet contact angles. The contact angles of HA coating and Ag-HA coating are 17.3˚ and 26.7˚ respectively, indicating that the coating has good hydrophilicity. The results of the bonding strength between the coating and pure titanium show that the HA coating is 27.4 MPa and the Ag-HA coating is 28.8 MPa, both of which are above 25 MPa. The results of the cell activity and attachment showed that the Ag-HA coating had better biocompatibility. The results of the OD value of bacteria on the Ag-HA coating is 0.16, and the OD value of the HA coating is 0.63, indicating that the addition of silver does have good antibacterial properties.