Gene-expression Profiling in a Patient with Esophageal Squamous Cell Carcinoma

發生在一位年輕食道癌病人的基因表現資料

許南榮(Nan-Yung Hsu) ； 江宜平(I-Ping Chiang) ； 施志勳(Chih-Shiung Shih) ； 陳品儒(Pin-Ru Chen) ； 何恆堅(Heng-Chien Ho)

《Mid-Taiwan Journal of Medicine》 10卷1期 (2005/03) Pp. 43-48

https://doi.org/10.6558/MTJM.2005.10(1).7

摘要

一位32歲男性病人有抽煙，喝酒和嚼檳榔10年以上的病史，因診斷在食道下段有鱗狀細胞癌而接受經食道裂孔切除和胃重建。根據病理檢查，腫瘤侵犯至表皮肌肉層，而無周圍淋巴腺轉移。於是，食道鱗狀細胞癌組織以cDNA微陣列方法，進行基因表現資料分析。一共篩選8354個基因，其中比正常組織多5倍螢光強度的基因表現有26個基因，而少5倍的有28個基因。經由資料搜查，發現26個基因中，僅有7個；而28個基因中，僅有8個已發表在文獻中。我們建議這些基因的特質可以針對具有抽煙，喝酒和嚼擯榔病史的年輕食道癌病人，提供食道鱗狀細胞癌惡化本質有用的資料。

關鍵字

食道鱗狀細胞癌 ； 微陣列

並列摘要

A 32-year-old man who has been an active tobacco smoker, alcohol drinker and betel nut chewer for more than 10 years was diagnosed as having esophageal squamous cell carcinoma (ESCC) after undergoing transhiatal esophagectomy and reconstruction with gastric graft. The pathology report indicated that the tumor had invaded the superficial muscle layer but had not metastasized to the adjacent lymph nodes. Gene-expression in the ESCC tissue was profiled by the cDNA microarray method. A total of 8354 genes were screened. Among them, 26 genes whose expression levels (fluorescence intensity) in esophageal carcinoma were more than 5 times (up regulated) those expressed in normal esophageal epithelium were selected; in addition, 28 genes whose expression levels in esophageal carcinoma were less than 0.2 times (down regulated) those expressed in the normal esophageal epithelium were selected. A search on MEDLINE of papers published from 1965 to the present revealed that only 7 of the 26 up-regulated genes and only 8 of the 28 down-regulated genes had been reported. We suggest that these characteristic genes will provide useful information for understanding the malignant nature of ESCC occurring in a young patient who has actively consumed tobacco, alcohol, and betel nut.

並列關鍵字

esophageal squamous cell carcinoma ； microarray