大花咸豐草超臨界流體萃取物抗氧化、抗發炎能力研究

A Study of the Antioxidant and Anti-Inflammatory Potential of Supercritical Carbon Dioxide Extract of Bidens pilosa

陳薪喨(Shin-Liang Chen) ； 林昀蓉(Yung-Rong Lin) ； 王蓉敏(Rong-Min Wang) ； 周培萱(Pei-Hsuan Chou) ； 陳美慧(Mei-Hui Chen) ； 黃敏鳳(Min-Feng Huang) ； 洪彰岑(Chang-Tsen Hung) ； 林榮宗(Rong-Tzong Lin) ； 林文傑(Wen-Jye Lin)

《加馬》 42期 (2011/06) Pp. 9-17

https://doi.org/10.30173/JG.201106.0002

摘要

大花咸豐草為菊科多年生植物，在傳統醫學上具有解熱、消炎與鎮痛的效果，所以用來治療氣管炎、肺炎、咽喉炎、痢疾等各種疾病。本研究將探討超臨界二氧化碳萃取大花咸豐草萃取物(BP)對於抗氧化、抗發炎之成效。實驗以總多酚、總類黃酮測定、DPPH自由基清除率、TEAC當量測定、鐵離子螯合和還原力測定等化學方法測試細胞外抗氧化能力，並以過氧化氫(H2O2)毒殺作用的保護能力測試細胞內抗氧化效果。另外以一氧化氮分析(NO assay)和硝基藍四氮還原試劑(NBT assay)評估萃取物抗發炎能力。實驗結果顯示BP的總多酚當量為79±0.25GAE(mg)/100g，總類黃酮當量為24.1±0.36 RE(mg)/100g，DPPH 50%自由基清除濃度(IC50)為25μg/mL，該濃度的TEAC當量為74±2.88μM。當BP濃度在350μg/mL時，對於H2O2的傷害有最大保護能力，約有70%的存活率，NO生成當量約38μM，硝基藍四氮(NBT)還原測試，發現可抑制25%細胞產生發炎反應。以超臨界流體二氧化碳萃取大花咸草效率高產量多且能保持其抗氧化、抗發炎特性。

關鍵字

超臨界二氧化碳萃取 ； 大花咸豐草 ； 抗氧化 ； 抗發炎

並列摘要

Bidens pilosa are Asteraceae perennial plant found widely throughout Taiwan. It is used as a folk medicine for the treatment of bronchitis, pneumonia, pharyngitis, dysentery, and the ingestion of poisons, on the basis of its antifebrile, antidotal, and analgesic effects. Supercritical Carbon Dioxide Extraction was used to obtain effective extracts from Bidens pilosa (BP). The total phenolic content, total flavonoid content 1, 1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity, Trolox equivalent anti-oxidizing capacity (TEAC) assay, ferrous ion chelating and reducing power were used to evaluate the overall antioxidative ability of the extracts. Nitric oxide and nitro blue tetrazolium (NBT) assays were used to evaluate anti-inflammatory properties. The results revealed that BP has a total phenolic content of 79±0.25 gallic acid equivalents (GAEs) mg/100g, total flavonoid content of 24.1±0.36 rutin equivalents (RE) mg/100g, DPPH radical scavenging activity of 50% (IC50) was 25 μg/mL, and an antioxidant activity of 74±2.88 μM Trolox equivalents. BP at a concentration of 350 μg/mL could prevent oxidative stress, induced by hydrogen peroxide (H2O2), and inhibit 25% of Phorbol esters (PMA) induced THP-1 monocyte differentiation.

並列關鍵字

Bidens pilosa ； Supercritical Carbon Dioxide Extraction ； antioxidant ； anti-inflammatory