This paper uses finite element method to study the stiffness of diamond motorcycle frame and its relationship with the natural frequencies. We derive the calculation formula for dynamic stiffness. 3D Drawing software Solid Works is used to draw geometry of the motorcycle frame which then input to the finite element software ANAYS/Workbench to build the simulation model. Experiment modal analysis is used to verify and revise the finite element model. We calculate the torsional stiffness, longitudinal bending stiffness, as well as lateral bending stiffness of the frame without and with engine. By changing the thickness and diameter of the frame tube, and observing the change of the natural frequencies, we can find their influence on the increase stiffness of the diamond frame.