According to the ASME B89.3.4 and the ISO 230-7, we developed a LabVIEW program to analyze the spindle error motion. Three laser triangulation displacement sensors were used to measure the special displacement of the spindle which was installed in the machinery. By analyzing the axial and radial error motion signals, the synchronous and the asynchronous error motion values were calculated as results. This technology could be regarded as the standard of the spindle metrology and the dynamic measurement accuracy evaluation.