The present paper intends to study comprehensibility estimates of public information symbols for screening the candidate symbols in ISO standardization procedure. A set of forty-four hospital symbols are used as the stimulus material, and 228 student subjects participated in this study. 95 male student subjects came from Chang Gung University and 133 female subjects came from Chang Gung Institute of Nursing. After a matching test and the comprehensibility estimates of each symbol, the correct match rates and the comprehensibility estimates are analyzied. The results showed that the approach presented in this study to derive membership functions is clearly worthy of further research.