Tributyltin Degradation in Estuarine Sediments and the Isolation of Tributyltin-Resistant Bacteria from These Sediments

探討三丁基錫在河口底泥中之降解及從底泥分離抗三丁基錫之菌株

劉秀美(Shiu-Mei Liu) ； 洪慧婷(Hui-Ting Huang) ； 夏夢珮(Meng-Pei Hsia) ； 張晉峰(Chin-Feng Chang)

《臺灣水產學會刊》 35卷4期 (2008/12) Pp. 315-327

https://doi.org/10.29822/JFST.200812.0002

摘要

本研究之目的旨在了解與確定河口沉積物中有機錫化合物之變化，以及發展清除底泥中有機錫化合物的技術。於此報告我們探討在有氧和無氧以及白天與黑夜下，於河口底泥中，生物和非生物性降解三丁基錫的情形。我們發現當三丁基錫被降解時，同時有二丁基錫、單丁基錫與無機錫的產生。三丁基錫在底泥中之最高降解率(0.052-0.030μM day^(-1))是高於它們在對照組無菌底泥中之降解率(0.009-0.013μM day^(-1))，因此在底泥中三丁基錫之降解機制主要是生物性降解作用。底泥中，生物性降解速率又以有氧狀態(0.092-0.130μM day^(-1))高於厭氧狀態(0.052-0.071μM day^(-1))。不過無論在有氧或是無氧狀態，在白天與夜晚之降解速率相近。我們從河口底泥中分離28株具三丁基錫抗性的菌株，它們皆是格蘭氏陰性桿狀菌。根據它們的16S rRNA序列分析，這些菌株可分為四大類，不過它們皆屬於γ-proteobacteria。

關鍵字

三丁基錫 ； 底泥 ； 降解 ； 菌株

並列摘要

In order to determine what happens to organotin compounds in estuarine sediments and to develop clean-up technologies for these compounds, this study investigated biotic and abiotic degradation of tributyltin (TBT) in estuarine sediment slurries under aerobic and anaerobic conditions, both in daylight and in darkness. Following the TBT degradation in estuarine sediment slurries, we found that dibutyltin (DBT), monobutyltin (MBT), and inorganic tin (Sn) were produced simultaneously. The highest TBT degradation rates in sediment slurries (0.052-0.130 μM day^(-1)) were much higher than those in aseptic control sediment slurries 0.009-0.013 μM day^(-1)). Thus, the biotic process was the most significant mechanism for TBT degradation in these estuarine sediment slurries. It was found that TBT degraded faster under aerobic conditions (0.092-0.130 μM day^(-1)) than under anaerobic conditions (0.052-0.071 μM day^(-1)) in sediment slurries. There were no significant differences in the highest TBT degradation rates, either in daylight or in darkness, under both aerobic and anaerobic conditions. Twenty eight TBT-resistant bacterial strains were isolated in this study, with all of the isolates being Gram negative, rod-shaped bacteria. The 16S rRNA gene sequence analyses revealed that these isolates could be categorized into four groups, but that they were phylogenetically closely affiliated to γ-proteobacteria.

並列關鍵字

degradation ； tributyltin ； estuarine sediment ； isolation ； tributyltin-resistant bacteria