The domestic seeds of wood oil tree, soapnut tree, Chinese tallow tree and camphor tree were examined in the following aspects: consist of fruits, quality of chemical and physical properties of oil, the seed-oil-production of fatty acid methyl ester (bio-diesel) and the by-products (glycerin). We found: (1) In terms of the yield of bio-diesel, the seeds of Chinese tallow tree and wood oil tree had the highest output (about 24.2-24.7%), the camphor tree was the middle one (about 22.6%), and soapnut tree had the lowest (about 4%). (2) All the four seeds can finish the transesterification improper conditions. The seeds of Wood oil tree, soapnut tree and camphor tree had above 98% therate of the transesterification, while Chinese tallow tree only had 62.9%. Besides, the kinds of bio-diesel from the different seeds differed in amounts, cold filter plugging point, iodine value, properties, transesterification process and cost. To sum up, according to this study, soapnut tree and wood oil tree are the optimal choices as bio-diesel raw materials.