N^6-Benzyl adenine對春石斛芽體增殖及發根後植株生育之影響

Effect of N^6 -Benzyl Adenine on Shoot Proliferation and Seedling Growth after Rooting Culture of Nobile-type Dendrobium

張珈錡(Jia-Ci Chang) ； 廖玉珠(Yu-Ju Liao) ； 鍾文全(Wen-Chuan Chung)

《植物種苗》 13卷4期 (2011/12) Pp. 35-46

https://doi.org/10.30077/SN.201112.0003

摘要

本研究以春石斛Nobile-type六個雜交品種經繼代培養2個月後之芽體基部，培養於含有0－5mg／L BA（N^6-Benzyladenine）之培養基中，結果顯示以3mg／L BA芽體增殖效果較佳，且無叢生狀畸形芽體產生。比較六個雜交品種在含有3mg／L BA之培養基中的芽體增殖倍數，得知以Den. Lai's Yukisakura之增殖倍數最高，達3.6倍，Den. Lai's New Yukidaruma最低為2.1倍。六個雜交品種經發根培養2個月後，發根率皆達100%，然各雜交品種間之植株生長卻呈顯著差異，各品種之培殖體平均葉數分別在2.7－3.8片之間，株高在4.3－6.1cm之間，根數在3.1－6.8根之間，根長則在1.6－4.3cm之間。將六雜交品種瓶苗移出至網室種植，植株存活率可達81.3－100%。

關鍵字

春石斛 ； 芽體増殖 ； 微體繁殖

並列摘要

An efficient in vitro micropropagation method offers potential for seedling propagation efficiency of nobile-type dendrobium was established. In this study, a shoot proliferation medium supplemented with 0 to 5 mg/L BA (N^6-Benzyl adenine) was evaluated its effect on shoot proliferation of stem base section as explant. Among all concentrations of BA tested, the medium supplemented with 3 mg/L BA was the best for shoot proliferation with shoot proliferation rate of 2.1-3.6 based on different varieties. In addition, the rooting rate of six varieties was 100% after two months rooting in the rooting medium, whereas significantly differences were found in seedling growth between different varieties. The average leaf number, height, root number and root length of plantlets were 2.7-3.8, 4.3-6.1 cm, 3.1-6.8 and 1.6-4.3 cm, respectively, in six varieties. The rooted plantlets were transplanted to greenhouse for further growth. Results showed that those plantlets had 81.3-100% survival rate in a greenhouse.

並列關鍵字

nobile-type dendrobium ； shoot proliferation ； micropropagation