生物材料之表面性質對腮線細胞生長之影響

The Effect of Surface Properties of Biomaterials on Parotid Acinar Cells Growth

許原華(Yuan-Hua Hsu)

指導教授 ： 楊台鴻

共同指導教授 ： 陳敏慧(Min-Huey chen)

國立臺灣大學/工學院/醫學工程學研究所/碩士(2004年)

https://doi.org/10.6342/NTU.2004.00260

摘要

本研究的目的是探討生物材料的表面性質對腮腺細胞的影響，進而應用於組織工程上。組織工程需有生物材料當組織支架，因此材料的表面性質便顯得格外重要了。材料的表面性質包括表面電荷、親疏水性、表面結構和界面自由能等會影響吸附蛋白的組成，進而調控不同細胞之行為，例如貼附、攤平、增生、轉移及分化。
本實驗分為兩部分，包括材料的親疏水性及表面結構兩方面。我們首先製備不同親疏水性之材料，包括PVA, chitosan, EVAL, TCPS(控制組) 和PVDF，利用接觸角決定其親疏水性，接著我們用免疫螢光染色(細胞角質素及抗ˇ

關鍵字

細胞角質素 ； 耳下腺 ； 腮腺細胞 ； 層連蛋白 ； 澱粉酶生物材料 ； 親疏水性

並列摘要

The purpose of this work was to study the effects of surface properties of biomaterials on acinar cells of parotid gland for further applied on salivary gland tissue engineering.
Its well-known surface properties, such as surface charge, interface energy, surface chemical, and surface topography, have significant effects on conformation and amount of adsorbed proteins.
In fact, researchers have indicated that the wettability of biomaterials had biofunction, which strongly influence cell attachment and spread.
In order to investigate the effects of materials on the growth of acinar cells, this work included two parts: wettability of biomaterials and surface topography. First, the wettability of different biomaterials, including PVA, chitosan, EVAL, TCPS (control group) and PVDF, were evaluated by contact angle test.
Before seeding acinar cells on these materials, the phenotype of cells was tested by immunocytochemical stain with acinar cells specific cytokeratin and anti-?amylase. After growing on these materials the activity, morphology and function of acinar cells were evaluated.
In the second part of this study, base on the results of the first part, we only selected two hydrophobic materials, i.e. EVAL and PVDF. We make a porous surface structure. In our hypothesis, by surface modification, proper surface free energy for biology requirement could be obtained and could assist cell growth.
Preliminary results showed poratid acinar cells grew better, in terms of attachment, spreading, and proliferation, on hydrophobic materials than on hydrophilic ones. However, the function of acinar cells was not necessarily correlated with cell growth.

並列關鍵字

acinar cell ； parotid gla ； wettability ； biomaterial

參考文獻

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