銅奈米團簇鑲嵌之紙質螢光感測平台

Copper Nanocluster-Paper Laminated Filter-Free Fluorescent Sensing Platform

陳家琳(Chia-Lin Chen)

指導教授 ： 廖尉斯

國立臺灣大學/理學院/化學研究所/碩士(2016年)

https://doi.org/10.6342/NTU201602197

摘要

紙質感測器由於低成本、易取得、高生物相容性以及環保等特色，在近年成為一熱門的檢測工具。然而，將螢光檢測技術應用於紙型平台經常會遭遇高背景值的問題，限制螢光在紙型感測器上的應用。因此我們結合紙質、具有大斯托克斯位移的銅奈米團簇以及熱塑膜封裝等設計來克服高背景值的問題，藉由半胱胺酸的偵測來展現此整合裝置的實際應用。當偵測樣品內存在半胱胺酸時會與銅奈米團簇反應產生螢光的變化，半胱胺酸的硫醇官能基會分解銅奈米團簇，因此從肉眼上觀察到螢光的消失。紙質感測器可利用數位相機作為偵測器量測螢光的變化，而拍攝的數位影像再利用軟體進行定量分析。紅綠藍顏色模型可有效地將銅奈米團簇的紅色螢光訊號從藍綠色背景值分離出來，不需使用任何光學濾光片，減少對精密光學配備的需求，並縮小背景值對偵測的干擾。此紙質平台與熱塑膜封裝以及抗氧化試片結合，可以有效地增長裝置的使用壽命至20天，並增強紙質的機械強度使其不易破碎。這一整合設計呈現低成本且不需任何光學濾光片的螢光紙質感測器，可藉由後續影像處理有效地降低在紙質平台的背景值並延長裝置壽命。

關鍵字

紙型感測器 ； 銅奈米團簇 ； 螢光偵測 ； 斯托克斯位移 ； 紅綠藍顏色模型

並列摘要

Paper-based analytical device is a robust sensing platform due to their low cost, high availability, biocompatibility, and environmental friendlily properties. However, paper materials often exhibit a strong background signal under UV illumination, limiting their application with fluorescent detections. In this study, an approach combining paper material, copper nanocluster with a large Stokes shift, and thermoplastic lamination is utilized to resolve this problem without the requirement of expensive optical filter setups. The capability of this integrated device is established through cysteine detection by the use of synthesized copper nanoclusters. A digital camera is applied to capture fluorescence changes on the platform and a signal reduction via thiol group induced metal cluster etching is observed. Red-Green-Blue (RGB) color imaging analysis can effectively remove the fluorescence background in absence of optical filters, and the red emission signals from copper nanoclusters can be quantified. The incorporation of thermoplastic films and antioxidative sheets greatly improves device durability under ambient conditions and provides mechanical supports. This integrated design offers a durable, low-cost, optical filter-free, and highly-reduced background fluorescent sensing platform.

並列關鍵字

paper-based analytical device ； copper nanocluster ； fluorescence detection ； large Stokes shift ； RGB color model

參考文獻

1. Martinez, A. W.; Phillips, S. T.; Carrilho, E.; Thomas, S. W.; Sindi, H.; Whitesides, G. M., Simple telemedicine for developing regions: camera phones and paper-based microfluidic devices for real-time, off-site diagnosis. Anal. Chem. 2008, 80, 3699-3707.

2. Martinez, A. W.; Phillips, S. T.; Whitesides, G. M.; Carrilho, E., Diagnostics for the developing world: microfluidic paper-based analytical devices. Anal. Chem. 2010, 82, 3-10.

3. Peeling, R. W.; Holmes, K. K.; Mabey, D.; Ronald, A., Rapid tests for sexually transmitted infections (STIs): the way forward. Sex. Transm. Infect. 2006, 82, V1-V6.

4. Sia, S. K.; Kricka, L. J., Microfluidics and point-of-care testing. Lab Chip 2008, 8, 1982-1983.

5. Lopez Marzo, A. M.; Pons, J.; Blake, D. A.; Merkoci, A., All-integrated and highly sensitive paper based device with sample treatment platform for Cd2+ immunodetection in drinking/tap waters. Anal. Chem. 2013, 85, 3532-3538.