The era of IoT (Internet of Things) is coming, everything has a smart function, and any object can be identified and located, and can be operated and controlled in the remote system. RFID (radio frequency identification) is one of the important key technologies. Especially in hospitals, textile identification and cleaning management of surgical gowns, doctors and nurses' apparel, patient's apparel and bed sheets, UHF (Ultra High Frequency) RFID can be read from a long distance, and it can prevent the launderers from contact infection during inventory. Conventional RFID chips and antennas are not resistant to washing and high temperature and high pressure sterilization. In this study, we will use waterproof and adhesive coating, combined with yarn and high temperature resistant polymer coating, to simulate medical washing test. UHF RFID yarns can be developed that are resistant to washing and high temperature and high pressure sterilization.