唐菖蒲萎凋病病原菌之選擇性培養基

Development of a Selective Medium for Detecting Fusarium oxysporum f.sp. gladioli

陳昱初(Y. C. Chen) ； 謝岱儒(T. J. Hsieh) ； 謝文端(W. H. Hsieh)

《植物病理學會刊》 14卷4期 (2005/12) Pp. 251-256

https://doi.org/10.6649/PPB.200512_14(4).0004

摘要

唐菖蒲萎凋病菌(Fusarium oxysporum f.sp. gladioli)選擇性培養基每公升含L-asparagine，2g；D-galactose，20g；MgSO4•7H2O，0.5g；NaB4O7•10H2O，1g；K2HPO4，1g；KCl，0.5g；Fe(EDTA)，5mg；oxgall，0.5g；PCNB (pentachloronitrobenzene)，1g；Benomyl (50% WP)，1g；streptomycin sulfate，0.3g；Agar，20g成份，以10%磷酸(phosphoric acid)溶液調整酸鹼值，不同酸鹼值之培養基具不同之功用。pH 4.0時可抑制唐菖蒲萎凋病菌除外之所有供試菌株孢子發芽，同時只有唐菖蒲萎凋病菌與百合萎凋病菌(F. oxysporum f.sp. lilii)之菌絲可正常生長，但兩者之菌落形態明顯不同，在pH 2.0時僅唐菖蒲萎凋病菌菌絲可以生長。以pH 4.0之培養基分離人工製作之厚膜孢子病土，其回收率可達96%以上，最低密度可偵測到每克土壤中含有50個繁殖體。以pH 2.0之培養基分離唐菖蒲罹病植株及球莖組織可於三天內偵測出病原菌。

關鍵字

唐菖蒲萎凋病 ； 選擇性培養基 ； 回收率 ； 最低偵測密度

並列摘要

A medium selective for isolation of Fusarium oxysporum f. sp. gladioli was developed by amending the Komada medium with 0.1% benlate. The medium supported good growth and high spore germination rate of F. oxysporum f. sp. gladioli, but strongly inhibited the growth and germination of 7 other formae speciales and 28 saprophytic Fusarium oxysporum. At pH 4, the medium completely inhibited spore germination of all isolates F. oxysporum tested but not F. oxysporum f. sp. gladioli. Only F. oxysporum f. sp. gladioli and F oxysporum f sp. lilii were capable of growth but with distinct colony morphology on this medium. F. oxysporum f. sp. gladioli was the only fungi tested capable of growth on the medium at pH 2. The modified Komada medium at pH 4 recovered 96% of condia of F. oxysporum f. sp. gladioli added to soil, and the medium at pH 2 was capable of detecting the pathogen in gladiolus corms within 3 days.

並列關鍵字

Fusarium oxysporum f. sp. gladioli ； selective medium ； pathogen detection