以陰離子選擇性徹底注射-掃略式電層 析技術分析食品中兒茶素

Anion-Selective Exhaustive Injection-Sweeping Electrokinetic Chromatographic Method for the Analyses of Catechins in Foods

黃意雲(I-Yun Huang)

指導教授 ： 黃悉雅

中原大學/理學院/化學研究所/碩士(2006年)

https://doi.org/10.6840/cycu200600658

摘要

兒茶素，一種普遍的存在於茶、葡萄汁及紅酒中的物質，近年來
的研究指出兒茶素能夠治療癌症，是一種對人體有益的物質。本研究
為結合陰離子掃略式徹底注射(ASEI-Sweeping)技術與微乳化電層析
(MEEKC)技術，發展出陰離子掃略式徹底注射-微乳化電層析
(ASEI-Sweeping-MEEKC)放大技術。此技術克服了過去微乳化電層析
在其他放大技術中因為穩定度不夠而導致放大不佳的瓶頸，並且我們
改變了前端溶液的注射長度及離子濃度，得到不錯的放大效果，另
外，我們還發現了變換微乳化溶液的組成，例如油相及共界面活性劑
的種類及比例，可以得到不同的放大分離效果，並且對於分析物有不
同的選擇性發生，證明了油相種類的不同對於放大效果是有明顯的影
響，此外，我們也改變了樣品的基質，發現在不同的pH 值環境下，
放大的結果有明顯的不同。
最後，由實驗的結果可以發現，使用這個放大的技術能夠有效的
降低偵測極限，另外，將此技術應用在檢測實際樣品上，可以有效偵
測到食品中所含微量兒茶素的種類及含量。

關鍵字

電層析 ； 兒茶素

並列摘要

In recent years, particular attentions have been given to catechins
because they can prevent from cancer. Since foods (such as tea, grape
juice and wine) are sources of a variety of catechins, thus a diet that is
good for health. Therefore, the analyses of catechins in foods were very
important.
In this study, an Anion-Selective Exhaustive Injection-Sweeping
MEEKC (ASEI-Sweeping-MEEKC) which was a combined technique of
Anion-Selective Exhaustive Injection-Sweeping (ASEI-Sweeping) and
MEEKC separation, was successfully established in order to improve the
detection limit of catechins for MEEKC method.
The length and composition of water plug and high-conductivity
solution (HCS) that presented in front of the capillary column were
examined for optimizing the ASEI-Sweeping method. In addition, the
composition of microemlusion solution (type and concentration of oil and
co-surfactant) were also demonstrated as efficient issues in concentration
enhancement. Furthermore, the sensitivity enhancement ratio (SER)
was markedly influenced by pH values of sample matrix.
Finally, the optimal ASEI-Sweeping MEEKC method was
successfully used for the analyses of trace catechins in various foods.

並列關鍵字

catechins ； electrokinetic chromatographic

參考文獻

11. 王鳳英譯，界面活性劑的原理與應用 (1986)

1. P.Camilleri, Capillary Electrophoresis Theory and Practice, Boca Raton, 1998.

2. J.W. Jorgenson, K.D. Lukacs, Science, 222, 266-272 (1983).

3. D.R. Baker, Capillary Electrophoresis ,New York 1995 21.

5. K.D. Altria, Journal of Chromatography A, 892, 171-186 (2000).