Genetic and Antimicrobial Susceptibility Variations of Staphylococcus aureus from Dairy Goat Raw Milk

由17個乳羊場生乳樣本分離之86株金黃色葡萄球菌(Staphylococcus aureus, S. aureus)依據生化特性、coa及nuc型別可將其區分為8個biotype、4個coa及4個nuc genotype，儘管三種方法分型結果有所差異，但發現乳羊場間最盛行的型別是biotype 2、coa genotype 2及genotype A。以16種抗微生物紙片進行敏感試驗，結果發現超過50%分離株對ampicillin, oxytetracycline, penicillin和tetracycline均具有抗藥性，然而發現所有分離株對methicillin, cephalothin, cefuroxime, enrofloxacin和bacitracin均有感受性，而對cloxacillin和sulfamethoxazole/trimethoprim則有低程度之抵抗性。接近80%之分離株有多重抗藥性情形，其中tetM是被發現主要對抗tetracycline之抗藥性基因。總結，乳羊場生乳分離之金黃色葡萄球菌並無發現methicillin resistant菌株，但存在著多重抗藥性及一個主要基因型別於場間傳播。

關鍵字

抗微生物感受性；乳山羊；基因型；生乳；金黃色葡萄球菌

並列摘要

Eighty-six Staphylococcus aureus (S. aureus) isolates were collected from 17 dairy goat farms and were characterized as having eight biotypes, four coa genotypes and four nuc genotypes based on biochemical characteristics, coa genotyping, and nuc genotyping methods, respectively. Although the results of genotyping from these three methods differed, the most prevalent isolates detected were biotype 2, coa genotype 2, and nuc genotype A. The results of antimicrobial susceptibility tests to 16 antimicrobials showed that over 50% of isolates were resistant to ampicillin, oxytetracycline, penicillin, and tetracycline, whereas all were susceptible to methicillin, cephalothin, cefuroxime, enrofloxacin and bacitracin, and exhibited lower resistance to cloxacillin and sulfamethoxazole/trimethoprim. Nearly 80% of isolates exhibited multi-drug resistance (MDR). Gene tetM, rather then tetK, was mainly responsible for resistance to tetracycline. In conclusion, MDR S. aureus and one major genotype type circulated among dairy goat farms and no methicillin-resistant Staphylococcus aureus (MRSA) has been found.

並列關鍵字

Antimicrobial susceptibility ； Daily goat ； Genotypes ； Raw milk ； Staphylococcus aureus

國際替代計量

Genetic and Antimicrobial Susceptibility Variations of Staphylococcus aureus from Dairy Goat Raw Milk

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