泥炭苔對叢枝菌根菌繁殖之影響

Effects of Peat Moss Amendment on Propagation of Arbuscular Mycorrhizal Fungi

莊明富(Ming-Fuh Chuang) ； 程永雄(Yung-Hsiung Cheng)

《中華農業研究》 48卷4期 (1999/12) Pp. 64-70

https://doi.org/10.29951/JARC.199912.0006

摘要

叢枝菌根菌Glomus mosseae的繁殖一般採用河砂為栽培介質，是因河砂具有高透氣性與低肥份的特性，但缺點是保水性差及不具緩衝性，泥炭苔則具有高保水性的優點，因此本試驗目的是探討河砂中添加泥炭苔，提高叢枝菌根菌在宿主根系的繁殖效果。試驗首先將泥炭苔(l0%)添加方式分成混合或覆蓋兩種處理，供試宿主植物有百喜草、營多藤、玉米及甘藷等四種，結果除甘藷外，菌根形成率與孢子濃度都以混合處理者高於覆蓋處理者。將河砂混合1/5, 1/3, 1/2（體積／體積）的泥炭苔，或單獨使用河砂，探討對G. mosseae在百喜草根圈土繁殖之影響，結果顯示盆缽中完全為河砂者，菌根形成率與厚膜孢子數最高，隨著泥炭苔混合比例增加，菌根形成率與厚膜孢子數逐漸減低。泥炭苔採用覆蓋方式，定期調查G. mosseae在玉米根圈土的繁殖，顯示隨接種時間增加，菌根形成率與孢子數逐漸提高，至接種後第11週達到高峰，而後逐漸降低。由以上結果，顯示單獨以河砂為介質，所生產的孢子數最高，添加泥炭苔反而不利於菌根形成與孢子的產生，其可能的原因有待進一步探討。

關鍵字

泥炭苔 ； 盆缽繁殖法 ； 叢枝菌根菌

並列摘要

Sand is a good medium for inoculum production of vesicular-arbuscular mycorrhizal fungi, because of its high aeration and lower fertility, but it has poor water-conserved and buffering ability. In contrast, peat moss has high water-conserved ability, so we try to amend sand with peat moss to improve initial growth of host plants, and to increase colonization and spore of VAM fungi. Effect of 10% peat moss mulched or mixed with sand on colonization and spore production of G. mosseae are tested in four host plants of bahiagrass, intortum, corn, and sweet potato. Results indicated sand mixed with peat moss is better than those mulched with peat moss. Then, the effects of sand mixed with peat moss in ratio of 1/5, 1/3, 1/2 on propagation of G. mosseae in rhizospheric soil of bahiagrass were conducted. Results indicated that the amendment of peat moss in sand could improve initial growth of host plants, but colonization or spore production was the highest in treatment without peat moss amendment. When amended volume of peat moss was over 1/4, there was no spore in rhizospheric soil at 14 weeks after inoculation. Periodic investigation were conducted to monitor propagation of G. mosseae in the rhizosphere of corn, and results indicated that root colonization and spore production in soil gradually increased, and reached the highest at 11 weeks after inoculation, and then decreased. The results showed that spore production was the highest in sand without any amendment. Peat moss was not beneficial to colonization and spore production, if amended with sand.

並列關鍵字

Peat moss ； Pot culture ； Arbuscular mycorrhizal fungi