The purpose of this study was to investigate the effects of different pollination methods on seed set and seedling vigor of sesame. The following five methods of pollination were carried out during sesame flowing time: open pollination, artificial self-pollination, artificial cross-pollination, pollination limited with net bag, and pollination limited with paper bag. The capsules were collected after 30 days of pollination and statistical analysis was performed. There were significant differences between open pollination and other four pollination methods. Compared with artificial self-pollination, the other two pollination methods, artificial cross-pollination and open pollination, significantly increased 1000-grain weight. There was no difference in seed germination rates among five pollination methods. However, in seedling vigor, the dry weight, bud length and root length can significantly increase by using artificial cross-pollination and open pollination methods.