攝食複合乳酸菌粉及優酪乳對健康成人腸道菌相及致突變性之影響

Effects of probiotic supplement formula and yogurt administration on the intestinal microflora and mutagenicity of healthy adults

翁麗華(Li-Hua Weng)

指導教授 ： 游若篍

國立臺灣大學/生物資源暨農學院/食品科技研究所/碩士(2006年)

https://doi.org/10.6342/NTU.2006.00956

摘要

本試驗探討攝食添加Lactobacillus acidophilus, Lactobacillus rhamnosus, Lactobacillus plantarum, Bifidobacterium bifidum, Bifidobacterium breve, Streptococcus thermophilus, Lactococcus lactis及果寡糖之複合乳酸菌粉及添加Lactobacillus acidophilus, Lactobacillus lactis, Bifidobacterium lactis及Lactococcus lactis之優酪乳對兩組健康成人(共48位)腸道菌相、水分含量、pH值及致突變性之影響。
結果顯示，攝食複合乳酸菌粉可使受試者腸道中有益菌之雙叉桿菌顯著增加(p < 0.01)，而有害菌之產氣莢膜梭菌顯著降低(p < 0.001)，具改善腸胃道之功能。攝取複合乳酸菌粉後之檢體萃取液對S. typhimurium TA 98之致突變性降低48 % (p < 0.001)，對S. typhimurium TA 100之致突變性降低34 % (p < 0.001)，表示攝食複合乳酸菌粉可降低受試者腸道中致突變物之致突變性。
攝食優酪乳可使受試者腸道中有益菌之雙叉桿菌顯著增加(p < 0.05)，而有害菌之產氣莢膜梭菌顯著降低(p < 0.05)，具改善腸胃道之功能。攝取優酪乳後之檢體萃取液對S. typhimurium TA 98之致突變性降低41 % (p < 0.05)，對S. typhimurium TA 100之致突變性降低52 % (p < 0.001)，表示攝食優酪乳可顯著降低受試者腸道中致突變物之致突變性。

關鍵字

複合乳酸菌粉 ； 優酪乳 ； 雙叉桿菌 ； 產氣莢膜梭菌 ； 腸道菌相

並列摘要

This study was conducted to investigate the effect of intake probiotic supplement formula containing Lactobacillus acidophilus, Lactobacillus rhamnosus, Lactobacillus plantarum, Bifidobacterium bifidum, Bifidobacterium breve, Streptococcus thermophilus, Lactococcus lactis, and fructo-oligo-saccharides and yogurt containing Lactobacillus acidophilus, Lactobacillus lactis, Bifidobacterium lactis, and Lactococcus lactis on the changes of intestinal flora, water content, pH values and mutagenicity in healthy adults.
Results indicated that ingestion of the probiotic supplement formula significantly increased the colony counts of fecal bifidobacteria (p < 0.01), and decreased the growth of Clostridium perfringens (p < 0.001). These results suggested that the intake of probiotic supplement formula was beneficial in improving the intestinal function. The mutagenic activities of subjects’ fecal extracts against S. typhimurium strains TA98 and TA100 were significantly decreased 48 % (p < 0.001), and 34 % (p < 0.001), irrespectively. These results suggested that the intake of probiotic supplement formula decrease the mutagenicity of mutagens in the intestinal tract.
Another result indicated that ingestion of yogurt significantly increased the colony counts of fecal bifidobacteria (p < 0.05) and decreased the growth of Clostridium perfringens (p < 0.05). These results suggested that the intake of yogurt was beneficial in improving the intestinal function. The mutagenic activities of subjects’ fecal extracts against S. typhimurium strains TA98 and TA100 were significantly decreased 41 % (p < 0.05), and 52 % (p < 0.001), irrespectively. These results suggested that the intake of yogurt decrease the mutagenicity of mutagens in the intestinal tract.

並列關鍵字

probiotic supplement formula ； yogurt ； bifidobacteria ； Clostridium perfringens ； intestinal microflora

參考文獻

行政院衛生署。2002。國人膳食營養素參考攝取量。行政院衛生署。台北。

行政院衛生署。2006。中華民國九十四年死因統計結果摘要。行政院衛生署。台北。

商惠芳、劉珍芳、曾金章、王正怡、李玲玟、葉必立、高信義、鍾美玉、呂秀介、謝明哲。2000。優酪乳對人體腸道細菌相及血液生化分析之影響。中華民國營養學會雜誌。25: 159-169。

Abdelali H, Cassand P, Soussotte V, Koch-Bocabeille B, Narbonne JF. 1995. Antimutagenicity of components of dairy products. Mutat Res 331: 133-141.

Adhikari K, Mustapha A, Grun IU, Fernando L. 2000. Viability of microencapsulated bifidobacteria in set yogurt during refrigerated storage. J Dairy Sci 83: 1946-1951.