Lignocellulosic materials are renewable resources that can be directly or indirectly used for the production of biofuels and commodity chemicals. However, these applications are limited by the association with three main components of the plant cell wall, cellulose, hemicellulose and lignin. Therefore, pretreatment of lignocelluloses to disrupt their recalcitrant structures is necessary in order to increase the digestibility of the materials. The current research investigates the utilization of Napier grass(Pennisetum purpureum) as a feedstock to produce a value-added product-fermentable sugars. This research focused on the use of steam explosion as the pretreatment method. Steam explosion treatment of lignocellulosic material has been previously described to increase cellulose digestibility. The governing factors for the treatment of steam explosion are steam temperature and retention time. The two factors are combined into single variable, severity factor, log(R0) . A factorial design was used to understand the influence of three variables in the process, temperature, retention time and sample particle size. The effect of steam explosion pretreatment on enzymatic hydrolysis from Napier grass was studied using a statistically method based on experimental design.