研究蜆萃取物對於肝星狀細胞的影響

在歐美，造成肝纖維化主要原因為酒精性肝炎、病毒性(C型病毒)肝炎和非酒精性肝炎。相對地，在亞洲地區造成肝纖維化主要的原因為病毒性肝炎(C型病毒和B型病毒)；此外，肝纖維化還會更進一步轉變成為肝硬化或肝癌。造成肝纖維化最主要的因素是因為細胞外基質的堆積而導致肝功能的缺失，在其過程中扮演最重要角色是活化態的肝星狀細胞。當肝星狀細胞由靜止態轉變成為活化態時，細胞會開始增生且造成α-SMA及膠原蛋白的大量表現。亞洲地區，蜆被廣泛認為對於肝損傷具有保護的作用，此篇論文即是探討蜆萃取物對於肝纖維化是否具有保護療效。根據動物實驗，於大鼠灌注四氯化碳9週，再給與蜆萃取物4週，經由組織病理分析其結果顯示蜆萃取物具有修復肝臟損傷的功效。另外，細胞實驗結果顯示，蜆萃取物可以減少活化態肝星狀細胞的增生並促使細胞停滯於G1/G0期。我們亦發現蜆萃取物會減少活化態肝星狀細胞α-SMA和第一型膠原蛋白的表現，此結果顯示蜆萃取物可能具有抑制活化態肝星狀細胞的作用。

關鍵字

肝星狀細胞；蜆；肝纖維化

並列摘要

In the western world, the major causes of liver fibrosis include alcohol, chronic hepatitis C infection and NASH (non-alcoholic steatohepatitis). This contrasts with Asia and Africa where chronic viral liver disease (hepatitis B and C) causes a massive burden of liver disease and cirrhosis. Furthermore, rates of hepatocellular carcinoma are high in these areas as cirrhosis predisposes to liver cancer. Liver fibrosis is characterized by excess deposition of collagens, resulting in an impairment of liver function and finally organ failure. Liver fibrosis is characterized by an activation of hepatic stellate cells (HSC). Quiescent HSCs become an activated phenotype which is characterized by alpha-smooth muscle actin (α-SMA) upregulation, increase in cell growth, and extracellular matrix secretion. The freshwater clam (Corbicula fluminea) is a widely-consumed shellfish and is used as a remedy for liver injury and anti-alcoholic toxicity in Asia. The purpose of this study would like to determine the roles of hepatoprotective effect of clam extracts. We have set up the intragastric infusion Tsukamoto-French rat model for approach in human liver fibrosis. According to our results of in vivo experiments, the clam extracts have ability to repair the injured by carbon tetrachloride in liver. In vitro assay, clam extracts significantly decreased activated HSC proliferation and induced an increase in the number of activated HSC in the G0/G1 phase by flow cytometry. In addition, clam extracts manifestly decreased α-SMA and collagen type I expression by Western blot assay in activated HSC. These results suggest clam extracts could inhibit activation of HSC.

並列關鍵字

clam extracts ； cell cycle ； α-SMA ； hepatic stellate cell ； HSC

參考文獻

行政院衛生署統計室 (2007). 民國95年台灣地區主要死因統計結果分析.

林志生 (2003). 飲用蜆萃出物對酒精性肝臟損傷之影響. 中華民國營養學會雜誌 28, 26-33.

陳陽德 (2007). 節水養蜆系統的生態調查與台灣蜆的濾食研究, 國立臺灣大學生命科學院漁業科學研究所碩士論文, ed. (台北).

Abergel, A., Sapin, V., Dif, N., Chassard, C., Darcha, C., Marcand-Sauvant, J., Gaillard-Martinie, B., Rock, E., Dechelotte, P., and Sauvant, P. (2006). Growth arrest and decrease of alpha-SMA and type I collagen expression by palmitic acid in the rat hepatic stellate cell line PAV-1. Dig Dis Sci 51, 986-995.

Abukhdeir, A.M., and Park, B.H. (2008). P21 and p27: roles in carcinogenesis and drug resistance. Expert Rev Mol Med 10, e19.

國際替代計量

研究蜆萃取物對於肝星狀細胞的影響

全文下載

主題瀏覽