The present paper main purpose proposes one gauging animal sensation of pain way. It can the quantification analyze the acupuncture to vibrate the change in the moth skin surface muscle. Using Optical triangle Measurement. Uses CMOS image sensor, the laser beam and tools and so on MATLAB software analysis successfully. Moth animal pattern which selects in view of us. Gauges her muscle to vibrate the change the state pattern. Obtains each kind of different stimulation separately (spatial acupuncture, 12% Formalin reagent, 37% Formalin reagent) frequency. But about obtains the frequency and the animal ache relatedness and the application, must in deep of the research.