電滲力應用於細胞/粒子收集效率之探討

An Investigation on the Collecting Efficiency of Cells and Micro Particles by Electro-osmosis

許哲維(Che Wei Hsu)

指導教授 ： 魏哲弘

大同大學/工程學院/機械工程學系所/碩士(2007年)

摘要

本論文主要是研究在兩平行電極中，使用交流電場來產生電滲力來進行酵母菌細胞 的收集。本研究利用康寧1737 的玻璃為底材濺鍍上鉻來當作底部電極，之後再塗佈 上一層薄膜正光阻作為絕緣層，利用微影技術在絕緣層上製造窗口，露出底部鉻電 極，而膠乳粒子及細胞則被收集於底部鉻電極上面打開的窗口，窗口大小約為 900μ m×900μ m , 依須求可打開數個窗口來進行收集，上電極使用ITO 玻璃作為電 極，以利於使用顯微鏡觀察實驗情況，二個電極間使用PDMS spacer 來隔開建立約 750μ m 距離。實驗使用酵母菌細胞，電流源採用方波來產生交流式電動力，施加電 壓為1-5Vp-p ，頻率為50-5000Hz 間，如此形成電動力式的微幫浦，進行對細胞的收 集。並同時採用商用軟體Femlab 3.0 電場的模擬分析，可瞭解電場分佈情形，進而 與實驗結果比較。此收集技術相當容易控制，對微型總合分析系統( μ TAS、 micro-Total Analysis Systems)及微流體技術未來也許有重大的影響和發展，並且可以 相當容易的和許多利用電場來進行分析的分析模組結合。

關鍵字

電動力、交流式電滲力、介電泳

並列摘要

The collecting and sorting yeast cells and latex particles by electric force is easy to
integrate with other bioassays. There are many forms of electric forces such as
electrophoresis, dielectrophoresis and electro-osmosis which can be used to manipulate
particles. In an attempt to understand the role of electro-osmosis and dielectrophoresis in
the collection of yeast cells and latex particles, a small device made of two parallel plates
is used to study the particle movement under low frequency AC condition. The device is
fabricated by a top electrode and a bottom electrode separated by a spacer. The top
electrode is made from an ITO glass and the bottom electrode is made from Corning 1737
glass sputtered with chromium. The spacer is made by spin coating a thin photo-resist
(0.5~1 μ m) to separate these electrodes. A 900μ m× 900 μ m collecting window is
defined on the bottom electrode by photolithography. A low frequency (50 Hz), low
power (5V) was applied to the top electrode to generate the non-uniform electric
field.There seems to be a range of frequency that leads to better particle movements. A
numerical simulation about electric field and fluid velocity distribution is used to
demonstrate the effect of dielectrophoresis and electro-osmosis.

並列關鍵字

dielectrophoresis ； electro-osmosis

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