Background: The role of a nursing practitioner enhances both diversity and autonomy of the nursing profession. However the newly transferred nursing practitioner faces the pressure towards the new role, and it is easy to cause exhaustion and even the possibility of errors if it is not adjusted properly. Objective: To explore the pressure nursing practitioner encounter when adjusting towards the new role. Methods: The qualitative content analysis was used and data was collected from interviewing ten nursing practitioners. Results: The pressure from the transition encountered by nursing practitioner mainly comes from personal pressure, professional and inter-professional pressure, as well as organizational management and expectations; the coping strategies include "external stress relief" and "internal adjustment". Conclusions and practical application: The novice nursing practitioners require a role model to enhance his or her recognition of professional abilities in order to improve self-confidence, and hence better professional performance. The result of this study may serve as a reference for future school curriculum planning or hospital in-service training.