Background and Purpose: The abdominal draw-in maneuver (ADIM), which is a main exercise usually used in clinics, is a core stability exercise in which TrA is selectively contracted earlier than other muscles on the surface such as the rectus abdominis, external oblique (EO), and internal oblique (IO) muscles. It is difficult to carry out ADIM precisely with sufficient activation of TrA. Blood flow restriction (BFR) is a training method performing light-load exercise with reduced blood flow to achieve an effective strengthening result similar with heavy-load training. However, there are few evidences for BFR training on core muscles, especially for TrA. The purpose of this study was to explore the effects of ADIM combined with BFR on TrA strengthening in healthy adults. Methods: Thirty healthy adults (age ranged 18-34 years) were recruited in the study and randomly divided into two groups including ADIM combined with BFR (n = 15) and ADIM alone (n = 15) groups. Participants received strengthening of ADIM combined with BFR or ADIM alone for 20 minutes per session, twice a week for 4 weeks. The thickness of IO, EO, and TrA measured by ultrasonography, core endurance, strength and stability measured by endurance test, dynamometer and double leg lowering test were evaluated before (week 1), after 8-session training (week 4) and final follow-up (week 5). Results: There were significantly increment on thickness and activation ratio of TrA and improvements on core endurance, strength and stability in the group trained with ADIM combined with BFR when compared to those with ADIM alone (p < 0.05). Conclusion: ADIM combined with BFR might strengthen TrA effectively and precisely in young healthy subjects. Clinical Relevance: The findings provide a potential clinical rehabilitation tool for training of core deep muscle, especially for TrA strengthening.