Effects of Caffeine on the Development of Deciduoma and Fetus

Sheng-Huang Chang ； Jen-Hsiang Tsai ； Chin-Chiu Tsai ； Yi-Fong Wang ； Je-Chiuan Ye ； Jer-Yuh Liu

《Adaptive Medicine》 14卷 (2022/03) Pp. 19-23

https://doi.org/10.4247/AM.2022.ACC264

摘要

Studies have discovered that caffeine readily crosses the placental barrier, thereby inducing undesirable effects early pregnancy events; however, the effects of caffeine on decidual formation, embryo implantation, and conceptus development have yet to be investigated. In this study, the direct influence of different caffeine doses on uterine decidualization in pseudopregnant rats and on the development of rat fetus and snail embryos was investigated. One of the two uterine horns was treated with different doses (0.5, 2, and 4 mg) of caffeine or antihistamine, and the other horn was treated with saline solution for use as a control. After 72 hours, the effects of caffeine on decidual development in the uterine horns were negligible, whereas the antihistamine significantly inhibited decidual development (p ＜ 0.05). In a subsequent test, naturally pregnant rats were administered high dosages of caffeine (60 mg/kg/day) or instilled saline solution for use as a control on gestation days 6 through 10. On gestation day 11, the rats were sacrificed, and their fetuses were examined. The differences in fetus count or weight between the two groups were nonsignificant. For the toxicity test, Physa Acuta snail embryos were treated with 0.3 and 0.45 mg/mL caffeine on development day (d-day) 2, decreasing the hatching rate to 58.51% and 16.30%, respectively, on d-day 12, whereas the two dosages on d-day 4 did not affect the hatching rate. Thus, although caffeine does not affect the development of deciduoma and the fetus, its effects in the later stages of embryonic development can be damaging.

關鍵字

caffeine ； decidualization ； pseudopregnancy ； pregnancy ； embryonic development