離心式微流體實驗系統的建置與 微流體驅動模式之研究

Establishment of Experimental System of Centrifugal Type Microfluid and Investigation of Microfluid Driving Model

廖威量(Wei-lianq Liaw)

指導教授 ： 陳夏宗

中原大學/工學院/機械工程研究所/博士(2004年)

https://doi.org/10.6840/cycu200400051

摘要

摘 要

「微流體驅動觀測系統」是研究微流道應用的生物晶片(Bio-chip)、實驗室晶片(Lab-on-a-chip)等所需的基礎設備，但現有流體系統多屬縮小製成的「固定式」微流體系統，常有洩漏、匹配等問題難以解決，且造價昂貴，以致遲遲沒有實用產品可上市。「離心式微流體系統」提供了另一個可能解決的方法與選擇，且相關文獻與實驗顯示其簡單、經濟、無洩漏等的特性與優點，在實用上極具優勢與潛力，非常值得開發與探討。
本研究中首先利用「提前補償觸發」原理與數位式PWM控制技術，配合能即時運算處理的單晶片微電腦「信號修正」電路與程式，成功的研製出能將旋轉中的影像清晰作同步「靜止」觀察的桌上型離心式微流體實驗系統--「閃碟2號」；也應用小型CCD與無線傳輸裝置，成功的研製出攜帶型的離心式微流體觀測裝置--「閃碟3號」。另外，本研究也利用「活塞氣囊」與「微流體光開關」的原理，分別開發出「離心式微氣輔裝置」與「微流體雷射計時裝置」，此兩裝置均可與「閃碟2號」和「閃碟3號」搭配而應用在微氣輔雙相流的研究或微量流體的定量研究上。而在微流道的設計與驅動模式方面，本研究也提出「平面驅動」理論，同時整理出離心式微流道設計的基本準則，對生醫產業或後續研究者提供極具參考與應用的價值。
經過初步的實驗與分析顯示，本文研製的各項裝置與設計均能有效運作且有極佳的性能與效果，特別是在新型生物晶片設計的模型方面，建立了更完備的實驗技術與初步的離心式微流體理論分析，對微流體生物晶片進一步實用化與多樣化的發展，應有相當的助益。

關鍵字

實驗室晶片 ； 生物晶片 ； 離心式微流體系統 ； 微流體驅動觀測系統

並列摘要

ABSTRACT
Microfluidic-drive monitoring system is an essential device to research of microchannel applied Bio-chip and Lab-on-a-chip. However, the fluidic systems currently used are mostly of miniature “fixed” microfluidic system with common problems of leakage and matching, as well as high cost. As a result, practical products are not yet introduced. The centrifugal microfluidic system provides another feasible solution. Related literatures and experiments have proved its simplicity, economical, effectiveness, and non-leaking. With advantages and potential, the system is worth developing and studying.
This study, based on the concept of “advanced compensation trigger” and digital PWM control technology, developed a desktop centrifugal-microfluid experimental system – “Flash 2” that is able to observe the rotating images in still concurrently with single chip microcomputer “signal modification” circuit and program that allows real-time algorithm processing. This study also used small CCD and wireless transmission device to develop a portable centrifugal-microfluid monitoring device – “Flash 3”. Furthermore, this study, based on concepts of “piston airbag” and “microfluidic light switch”, developed a centrifugal micro gas-assisted device and a microfluidic laser timing device that could work together with Flash 2 and Flash 3 on micro air-assisted two-phase flow researches or quantitative microfluid researches. In terms of the design and drive model of microchannel, this study proposed an innovative “plane drive” concept, and compiled the principles of centrifugal microchannel design for reference to biopharmaceutical industry or future researches.
The experiment and analysis results show that the devices and design developed by this study are able to deliver superior performance and effect. The innovative Bio-chip design and experimental technology consistence with preliminary analysis of microfluidic theory are beneficial to the practicability and diversity of the development of centrifugal-microfluid Bio-chip.

並列關鍵字

Lab-on-a-chip ； Bio-chip ； centrifugal microfluidic system ； Microfluidic-drive monitoring system

參考文獻

9. Madou M. J., Lee L. J., Koelling K. W., Daunert Sylvia, “Design and Fabrication of Polymer Microfluidic Platforms for Biomedical Applications”, Ohio State University.

14. Peng X.F., and Peterson G.P., “The Effect of Thermofluid and Geometrical Parameters on Convection of Liquids Through Rectangular Microchannels”, International Journal of Heat and Mass Transfer, Vol.15, pp.755-758, 1995.

15. Peterson G.P., Swanson L.W. and Gerner F.M., “Micro Heat Pipes”, Advances in Heat Transfer, Vol.25, pp.295-337, 1995.

39. Roberson John A. and Crowe Clayton T. “Engineering Fluid Mechanics”, Houghton Mifflin Company, 1980.

46. Chang C. T., Chen S. C., Chen J. P., Huang L. T. and Peng H. S., 2000, “Drop-Impact Simulation of A 3D Thin-Wall Part”, 中華民國力學學會年會暨第24屆全國力學會議, Q-15, Chung Li, Taiwan.