應用RAPD分子指紋研究木賊葉木麻黃國際種源之遺傳變異

Genetic Variation in International Provenances of Casuarina equisetifolia (Casuarinaceae) Based on RAPD Fingerprinting

何坤益(Kuen-Yih Ho) ； 蕭如英(Ju-Ying Hsiao) ； 鄧書麟(Shu-Lin Deng)

《台灣林業科學》 17卷2期 (2002/06) Pp. 183-193

https://doi.org/10.7075/TJFS.200206.0183

摘要

In this study, the genetic variation and population structure of 244 individuals sampled from 19 provenances of Casuarina equisetifolia were examined using RAPD fingerprinting. Twelve primers were used which yielded 89 polymorphic bands. The analysis of molecular variance (AMOVA) revealed that the variance component between native and cultivated provenance groups was 5.64•(p＜0.001), among provenances within groups was 27.8•(p＜0.001), and among individuals within provenances was 66.52•(p＜0.001). Remarkable genetic differentiation and low levels of gene flow were detected between native provenances due to geographical isolation. In contrast, cultivated provenances had high gene flow and low genetic differentiation perhaps due to coancestry. The result of UPGMA cluster analysis revealed that the cultivated provenances might have originated of native provenances from Thailand(C26), Fiji(C19, C20), and Australia(C2). The groups of native provenances revealed geographic relationships among these islands.

關鍵字

層級性遺傳變方分析 ； 木賊葉木麻黃 ； 基因流傳 ； 遺傳分化 ； 逢機擴大多型性去氧核醣核酸分子指紋技術

並列摘要

In this study, the genetic variation and population structure of 244 individuals sampled from 19 provenances of Casuarina equisetifolia were examined using RAPD fingerprinting. Twelve primers were used which yielded 89 polymorphic bands. The analysis of molecular variance (AMOVA) revealed that the variance component between native and cultivated provenance groups was 5.64•(p＜0.001), among provenances within groups was 27.8•(p＜0.001), and among individuals within provenances was 66.52•(p＜0.001). Remarkable genetic differentiation and low levels of gene flow were detected between native provenances due to geographical isolation. In contrast, cultivated provenances had high gene flow and low genetic differentiation perhaps due to coancestry. The result of UPGMA cluster analysis revealed that the cultivated provenances might have originated of native provenances from Thailand(C26), Fiji(C19, C20), and Australia(C2). The groups of native provenances revealed geographic relationships among these islands.

並列關鍵字

AMOVA ； Casuarina equisetifolia ； gene flow ； genetic differentiation ； RAPD