Translated Titles

Effects of 4-Week Chlorella Supplementation on Resistance Exercise-Induced Muscle Damage in Athletes




祁崇溥(Chung-Pu Chi);張世沛(Shih-Pei Chang);楊孫錦(Sun-Chin Yang);林學宜(Shueh-Yi Lin);余思賢(Szu-Hsien Yu)

Key Words

疼痛 ; 肌酸激酶 ; 乳酸去氫酶 ; pain ; creatine kinase ; lactate dehydrogenase



Volume or Term/Year and Month of Publication

19卷2期(2017 / 06 / 30)

Page #

183 - 194

Content Language


Chinese Abstract

先前研究顯示短時間綠藻補充可以改善高強度阻力運動引起肌肉疲勞現象。本研究目的為以增加補充時間的方式,探討補充4 週綠藻,對運動後恢復期肌肉損傷與最大肌力表現的影響。研究方法為隨機雙盲安慰劑組實驗設計,將24 名羽球運動選手研究受試者分成安慰劑組與綠藻組。每位受試者皆完成口服28 天綠藻(4.5 克/天)或等量的安慰劑(澱粉)後,進行單次阻力運動以誘發肌肉損傷,損傷模式以最大肌力為負荷進行大腿推蹬、大腿曲屈與胸部推舉各進行各4 組。並於補充前、運動後24 與後48 小時分別測量血清肌酸激酶與乳酸去氫酶活性,並以量表評估肌肉疼痛情形,於運動後第3、4、5 天測量最大肌力表現。研究結果顯示:阻力運動造成血液肌酸激酶活性與肌肉痠痛上升,並造成肌肉力量降低。綠藻組運動後第24 及第48 小時血液肌酸激酶活性與肌肉痠痛顯著低於安慰劑組。綠藻組運動後3 至4 天大腿推蹬力量顯著高於安慰劑組,運動後4 至5 天大腿曲屈與胸部推舉力量顯著高於安慰劑組。綜合上述結果,本研究結論為較長時間的綠藻補充不但可以避免運動後肌肉疲勞,且會降低恢復期肌肉損傷。

English Abstract

Our previous study has shown that 7 days Chlorella powder pre-supplementation can attenuate the resistance exercise-induced muscle fatigue. The purpose of this study was to determine the effects of Chlorella supplementation for four weeks on exercise-induced muscle damage and maximal muscle strength during recovery. In this randomized, double-blind, placebo-controlled study, 24 college badminton players were recruited, and divided into the placebo or Chlorella trials. The subjects were supplemented with Chlorella powder (4.5 g/ day) or placebo for 28 days, and then challenged to an acute resistance exercises (5 sets of 1 RM, with a 1-min rest between sets) to induce muscle damage. The muscle damage and muscle soreness markers were measured at 24 and 48 hours after exercise challenge in both trials. Maximum muscle strength was determined on day 3, 4, 5 after acute exercise. The results showed that the blood creatine kinase, lactate dehydrogenase activities and muscle soreness were significantly increased, whereas muscle strength was decreased after acute resistance exercise in the placebo trial. However, creatine kinase activities and muscle soreness of participants in the Chlorella trial were significantly lowered compared with placebo trial at 24 and 48 hours following resistance exercise. Furthermore, the maximum muscle strength of leg press on 3rd and 4th day, and leg curl and chest press on 4th and 5th day during recovery period in Chlorella trial were significantly higher than the placebo trial. Our results conclude that Chlorella supplementation can reduce the resistance exercise-induced muscle damage, and decrease muscle fatigue in athletes during the recovery.

Topic Category 社會科學 > 體育學
  1. 祁崇溥、蔡震壽、趙振瑞(2013)。短期補充綠藻對運動後疲勞恢復的影響。大專體育學刊,15(3),348-358。
  2. An, H.-J.,Choi, H.-M.,Park, H.-S.,Han, J.-G.,Lee, E.-H.,Park, Y.-S.,Kim, H.-M.(2006).Oral administration of hot water extracts of Chlorella vulgaris increases physical stamina in mice.Annals of Nutrition and Metabolism,50(4),380-386.
  3. An, H.-J.,Seo, S.-W.,Sim, K.-S.,Kim, J.-S.,Kim, E.-H.,Lee, M.-O.,Kim, H.-M.(2006).Antifatigue effect of Chlorella vulgaris in mice.The Korean Journal of Food and Nutrition,19(2),169-175.
  4. Azizbeigi, K.,Azarbayjani, M. A.,Atashak, S.,Stannard, S. R.(2015).Effect of moderate and high resistance training intensity on indices of inflammatory and oxidative stress.Research in Sports Medicine,23(1),73-87.
  5. Brancaccio, P.,Lippi, G.,Maffulli, N.(2010).Biochemical markers of muscular damage.Clinical Chemistry and Laboratory Medicine,48(6),757-767.
  6. Chen, J.-L.,Yeh, D.-P.,Lee, J.-P.,Chen, C.-Y.,Huang, C.-Y.,Lee, S.-D.,Kuo, J.-H.(2011).Parasympathetic nervous activity mirrors recovery status in weightlifting performance after training.The Journal of Strength & Conditioning Research,25(6),1546-1552.
  7. Draganidis, D.,Chatzinikolaou, A.,Jamurtas, A. Z.,Carlos Barbero, J.,Tsoukas, D.,Theodorou, A. S.,Fatouros, I.(2013).The time-frame of acute resistance exercise effects on football skill performance: The impact of exercise intensity.Journal of Sports Sciences,31(7),714-722.
  8. Ebrahimi-Mameghani, M.,Sadeghi, Z.,Abbasalizad Farhangi, M.,Vaghef-Mehrabany, E.,Aliashrafi, S.(2017).Glucose homeostasis, insulin resistance and inflammatory biomarkers in patients with non-alcoholic fatty liver disease: Beneficial effects of supplementation with microalgae Chlorella vulgaris: A double-blind placebo-controlled randomized clinical trial.Clinical Nutrition,36(4),1001-1006.
  9. Fochi, A. G.,Damas, F.,Berton, R.,Alvarez, I.,Miquelini, M.,Salvini, T. F.,Libardi, C. A.(2016).Greater eccentric exercise-induced muscle damage by large versus small range of motion with the same end-point.Biology of Sport,33(3),285-289.
  10. Giessing, J.,Eichmann, B.,Steele, J.,Fisher, J.(2016).A comparison of low volume "highintensity-training" and high volume traditional res i s tance t raining methods on muscular performance, body composition, and subjective assessments of training.Biology of Sport,33(3),241-249.
  11. Gonzalez, A. M.,Hoffman, J. R.,Townsend, J. R.,Jajtner, A. R.,Boone, C. H.,Beyer, K. S.,Stout, J. R.(2015).Intramuscular anabolic signaling and endocrine response following high volume and high intensity resistance exercise protocols in trained men.Physiological Reports,3(7),e12466.
  12. Halperin, S. A.,Smith, B.,Nolan, C.,Shay, J.,Kralovec, J.(2003).Safety and immunoenhancing effect of a Chlorella-derived dietary supplement in healthy adults undergoing influenza vaccination: Randomized, double-blind, placebo-controlled trial.Canadian Medical Association Journal,169(2),111-117.
  13. Hidalgo-Lucas, S.,Bisson, J.-F.,Duffaud, A.,Nejdi, A.,Guerin-Deremaux, L.,Baert, B.,Rozan, P.(2014).Benefits of oral and topical administration of ROQUETTE Chlorella sp. on skin inflammation and wound healing in mice.Anti-Inflammatory & Anti-Allergy Agents in Medicinal Chemistry,13(2),93-102.
  14. Howatson, G.,Hough, P.,Pattison, J.,Hill, J. A.,Blagrove, R.,Glaister, M.,Thompsom, K. G.(2011).Trekking poles reduce exercise-induced muscle injury during mountain walking.Medicine and Science in Sports and Exercise,43(1),140-145.
  15. Howatson, G.,Van Someren, K. A.(2008).The prevention and treatment of exercise-induced muscle damage.Sports Medicine,38(6),483-503.
  16. Kim, J. Y.,Paik, J. K.,Kim, O. Y.,Park, H. W.,Lee, J. H.,Jang, Y. S.,Lee, J. H.(2011).Effects of lycopene supplementation on oxidative stress and markers of endothelial function in healthy men.Atherosclerosis,215(1),189-195.
  17. Kim, S.,Kim, J.,Lim, Y.,Kim, Y. J.,Kim, J. Y.,Kwon, O.(2016).A dietary cholesterol challenge study to assess Chlorella supplementation in maintaining healthy lipid levels in adults: A double-blinded, randomized, placebo-controlled study.Nutrition Journal,15(1),54.
  18. Klapcińska, B.,Iskra, J.,Poprzecki, S.,Grzesiok, K.(2001).The effects of sprint (300 m) running on plasma lactate, uric acid, creatine kinase and lactate dehydrogenase in competitive hurdlers and untrained men.Journal of Sports Medicine and Physical Fitness,41(3),306-311.
  19. Lee, S. H.,Kang, H. J.,Lee, H. J.,Kang, M. H.,Park, Y. K.(2010).Six-week supplementation with Chlorella has favorable impact on antioxidant status in Korean male smokers.Nutrition,26(2),175-183.
  20. Lin, C. Y.,Huang, P. J.,Chao, C. Y.(2014).Chlorella protects against hydrogen peroxideinduced pancreatic β-cell damage.Journal of Medicinal Food,17(12),1273-1280.
  21. Liu, J.,Chen, F.(2016).Biology and industrial applications of Chlorella: Advances and prospects.Advances in Biochemical Engineering/Biotechnology,153,1-35.
  22. Marcora, S. M.,Bosio, A.(2007).Effect of exerciseinduced muscle damage on endurance running performance in humans.Scandinavian Journal of Medicine & Science in Sports,17(6),662-671.
  23. Merchant, R. E.,Andre, C. A.,Wise, C. M.(2001).Nutritional supplementation with Chlorella pyrenoidosa for fibromyalgia syndrome: A double-blind, placebo-controlled, crossover study.Journal of Musculoskeletal Pain,9(4),37-54.
  24. Merchant, R. E.,Carmack, C. A.,Wise, C. M.(2000).Nutritional supplementation with Chlorella pyrenoidosa for patients with fibromyalgia syndrome: A pilot study.Phytotherapy Research,14(3),167-173.
  25. Miyazawa, T.,Nakagawa, K.,Takekoshi, H.,Higuchi, O.,Kato, S.,Kondo, M.,Miyazawa, T.(2013).Ingestion of Chlorella reduced the oxidation of erythrocyte membrane lipids in senior Japanese subjects.Journal of Oleo Science,62(11),873-881.
  26. Mizoguchi, T.,Arakawa, Y.,Kobayashi, M.,Fujishima, M.(2011).Influence of Chlorella powder intake during swimming stress in mice.Biochemical and Biophysical Research Communications,404(1),121-126.
  27. Mizoguchi, T.,Takehara, I.,Masuzawa, T.,Saito, T.,Naoki, Y.(2008).Nutrigenomic studies of effects of Chlorella on subjects with high-risk factors for lifestyle-related disease.Journal of Medicinal Food,11(3),395-404.
  28. Montgomery, P. G.,Pyne, D. B.,Cox, A. J.,Hopkins, W. G.,Minahan, C. L.,Hunt, P. H.(2008).Muscle damage, inflammation, and recovery interventions during a 3-day basketball tournament.European Journal of Sport Science,8(2),241-250.
  29. Nakashima, Y.,Ohsawa, I.,Nishimaki, K.,Kumamoto, S.,Maruyama, I.,Suzuki, Y.,Ohta, S.(2014).Preventive effects of Chlorella on skeletal muscle atrophy in muscle-specific mitochondrial aldehyde dehydrogenase 2 activity-deficient mice.BMC Complementary and Alternative Medicine,14
  30. Ojala, T.,Häkkinen, K.(2013).Effects of the tennis tournament on players' physical performance, hormonal responses, muscle damage and recovery.Journal of Sports Science and Medicine,12(2),240-248.
  31. Otsuki, T.,Shimizu, K.,Maeda, S.(2015).Changes in arterial stiffness and nitric oxide production with Chlorella-derived multicomponent supplementation in middle-aged and older individuals.Journal of Clinical Biochemistry and Nutrition,57(3),228-232.
  32. Otsuki, T.,Shimizu, K.,Zempo-Miyaki, A.,Maeda, S.(2016).Changes in salivary flow rate following Chlorella-derived multicomponent supplementation.Journal of Clinical Biochemistry and Nutrition,59(1),45-48.
  33. Pasiakos, S. M.,Lieberman, H. R.,McLellan, T. M.(2014).Effects of protein supplements on muscle damage, soreness and recovery of muscle function and physical performance: A systematic review.Sports Medicine,44(5),655-670.
  34. Potteiger, J. A.,Blessing, D. L.,Wilson, G. D.(1992).Effects of varying recovery periods on muscle enzymes, soreness, and performance in baseball pitchers.Journal of Athletic Training,27(1),27-31.
  35. Renju, G. L.,Kurup, G. M.,Saritha Kumari, C. H.(2014).Effect of lycopene from Chlorella marina on high cholesterol-induced oxidative damage and inflammation in rats.Inflammopharmacology,22(1),45-54.
  36. Sewright, K. A.,Hubal, M. J.,Kearns, A.,Holbrook, M. T.,Clarkson, P. M.(2008).Sex differences in response to maximal eccentric exercise.Medicine and Science in Sports and Exercise,40(2),242-251.
  37. Soares, S.,Ferreira-Junior, J. B.,Pereira, M. C.,Cleto, V. A.,Castanheira, R. P.,Cadore, E. L.,Bottaro, M.(2015).Dissociated time course of muscle damage recovery between single- and multi-joint exercises in highly resistance-trained men.The Journal of Strength & Conditioning Research,29(9),2594-2599.
  38. Umemoto, S.,Otsuki, T.(2014).Chlorella-derived multicomponent supplementation increases aerobic endurance capacity in young individuals.Journal of Clinical Biochemistry and Nutrition,55(2),143-146.
  39. Vijayavel, K.,Anbuselvam, C.,Balasubramanian, M. P.(2007).Antioxidant effect of the marine algae Chlorella vulgaris against naphthaleneinduced oxidative stress in the albino rats.Molecular and Cellular Biochemistry,303(1-2),39-44.
  40. Wells, A. J.,Hoffman, J. R.,Jajtner, A. R.,Varanoske, A. N.,Church, D. D.,Gonzalez, A. M.,Stout, J. R.(2016).Monocyte recruitment after highintensity and high-volume resistance exercise.Medicine and Science in Sports and Exercise,48(6),1169-1178.
  41. Willoughby, D. S.,McFarlin, B.,Bois, C.(2003).Interleukin-6 expression after repeated bouts of eccentric exercise.International Journal of Sports Medicine,24(1),15-21.
Times Cited
  1. 葉丁鵬,祁崇溥,林佳霈,余思賢(2019)。補充綠藻萃取物對於運動員脫水後運動疲勞之影響。大專體育學刊,21(2),185-198。