結合電紡與冷凍乾燥技術製備雙層多孔性皮膚支架

陳信宏

doi:10.6841/NTUT.2012.00720

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結合電紡與冷凍乾燥技術製備雙層多孔性皮膚支架

Preparation of a Bi-layer Porous Skin Scaffold Fabricated via Electrospinning and Freeze-drying Techniques

陳信宏 , Masters　　Advisor：林忻怡　　

繁體中文 DOI： 10.6841/NTUT.2012.00720

電紡；冷凍乾燥；奈米纖維；幾丁聚醣；聚乙烯醇；果膠；戊二醛；纖維母細胞； Electrospinning ； Freeze-drying ； Nanofiber ； Chitosan ； Polyvinyl Alcohol ； Pectin ； Glutaraldehyde ； Fibroblasts

Abstract │ Reference (89) │ Altmetrics

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In the present study, we used chitosan to make a bi-layered composite artificial skin construct for full-thickness skin repair. The epidermal layer was constructed from a nano-fiber matrix, and the dermal layer was similar to porous sponge structure. In this process, the dermal layer forms a built-in basal membrane. This built-in basal membrane can separate the different cells into two layers that better simulate natural skin structure. 
This bi-layered composite artificial skin scaffold has to cross-link with glutaraldehyde to stabilize the physical structure. In degradation test, this porous, nano-fiber structure can be maintained for more than 7 days, which is enough time to allow for skin growth and repair. In swelling test, the variation of swelling volume is within a narrow range and will not change with time to cause extrusion to the surrounding healthy skin. In tensile strength test, the mechanical strength of bi-layered structure is higher than the traditional single-layer and approach the natural skin. The cells will grow rapidly with time, and examination via electron microscope shows that these cells produce extracellular matrix (collagen).
The study results indicate that a bi-layered composite artificial skin structure is more similar than the traditional artificial structure to natural skin structure, and with better structural stability and mechanical strength, and with high cell compatibility which can induce cell growth into the scaffolds.

Reference ( 89 ) 〈TOP〉

Beele, H. (2002). "Artificial skin: past, present and future." International Journal of Artificial Organs 25: 163-173.
連結：
Bhardwaj, N. and S. C. Kundu (2010). "Electrospinning: A fascinating ﬁber fabrication technique." Biotechnology Advances 28: 325-347.
連結：
Buchko, C. J., L. C. Chen, et al. (1999). "Processing and microstructural characterization of porous biocompatible protein polymer thin films." Polymer 40: 7397-7407.
連結：
Deitzel, J. M., J. Kleinmeyer, et al. ( 2001). "The effect of processing variables on the morphology of electrospun nanofibers and textiles." Polymer 42: 261-272.
連結：
Formhals, A. (1934). "Process and apparatus for preparing artiﬁcial threads." U.S. Patent: No. 1975504.
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