新日本靈芝、環紋靈芝純培養菌絲體之生理性狀及核醣體DNA定序之研究

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新日本靈芝、環紋靈芝純培養菌絲體之生理性狀及核醣體DNA定序之研究

Cultural, Physiolocal and Ribosomal DNASequencing Studies ofGanoderma neojaponicumand G. zonatum

謝富貴 , Masters　　Advisor：葉增勇　　

繁體中文

靈芝；新日本靈芝；環紋靈芝；純培養菌絲體；核醣體DNA ； G. neojaponicum ； G. zonatum ； Ribosomal DNASequencing ； pure cultures

Abstract │ Reference (99) │ Altmetrics

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Ganoderma neojaponicum was obtained from Taiwan, while G. zonatum was obtained from U. S. A. Hence their pure cultures were used for morphological, physiological studies and ribosomal DNA sequencing analysis.
The morphological characters of G. neojaponicum and G. zonatum were both observed as the white color on the mycelial mat’s surface, brown color on the other side, clamped generative hyphae and the appearance of swelling cells in culture.
The positive reaction in extracellular oxidases which included laccase and peroxidase, but negative in tyrosinase, indicated G. neojaponicum and G. zonatum belong to white rotting fungi, and showed that they can utilize lignin and cellulose of wood。
The results of physiological tests were shown as follows:
1.Optimum temperature for the mycelial growth of G. neojaponicum was at 24~28℃ on both of MEA and PDA, while G. zonatum was at 32℃ both on MEA and PDA.
2. Optimum pH for the mycelial growth of G. zonatum was 4.6, while G. neojaponicum was not prominent.
3. Glucose concentration at 40~80g/L was the optimum condition for the mycelial growth of G. neojaponicum and G. zonatum. The increasing glucose concentration neither increased nor decreased mycelial growth rate of G. neojaponicum, but decreased mycelial growth rate of the G. zonatum.
4.Starch was the best carbon source for both mycelial growth of G. neojaponicum and G. zonatum.
3. Optimum nitrogen concentration of ammonium nitrate (NH4NO3) was at 0.02N for the mycelial growth of the G. neojaponicum, but at 0.04N for the G. zonatum. Whereas the mycelial growth of G. zonatum was in proportion to the concentration of L-Asparagine (organic nitrogen form) , but not for G. neojaponicum.
Sequencing analyses of the ITS1-5.8S-ITS2 ribosomal DNA in G. neojaponicum are 195bp-158bp-201bp and in G. zonatum are 208bp-158bp-195bp respectively. The sequences data showed that G. zonatum was close to G.formosanum 0109 and G. neojaponicum. The phylogenetic relationship tree constructed  from their study will aid to realize the molecular evolution among Ganoderma spices.

Reference ( 99 ) 〈TOP〉

Adaskaveg, J. E. and R. L. Gilbertson. 1986. Cultural studies and genetics of sexuality of the G. lucidum and G. tsugae in relation to the taxonomy of the G. lucidum complex. Mycologia 78:700-711.
連結：
Chung-Yuh, Zeng-Yung Yeh, and Guey-Jen Lee-Chen. 1996. Analysis of Genetic Diversity of Two Intersterility Groups of Ganoderma australe by DNA Seqoencing.師大生物學報 31(1): 47-53。
連結：
Baura G., T.M. Szaro and T. D. Bruns. 1992 Gastrosuillus laricinus is a recent derivative of Suillus grevillei: molecular evidence. Mycologia, 84: 592-597.
連結：
Berbee, M. L., Yoshimura, A., Sugiyama J. and Taylor, J. W. 1995. Is Penicillium monophyletic? An evaluation of phylogeny in the family Trichocomaceae from 18S, 5.8S and ITS ribosomal DNA sequence data. Mycologia, 87: 210-222.
連結：
Bose, S. R. 1929. Artificial culture of Ganoderma lucidum Leyss. From spore to spore. Bot. Gaz. 87:665-667.
連結：

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