Bacterial Decolourization Enhancement Using a Constructed Mixed Consortium

「人工建構」混合菌之細菌脫色加強研究

陳博彥(Bor-Yann Chen) ； 張嘉修(Jo-Shu Chang) ； 陳姍玗(Shan-Yu Chen)

《Journal of the Chinese Institute of Chemical Engineers》 34卷5期 (2003/09) Pp. 513-524

https://doi.org/10.6967/JCICE.200309.0513

摘要

由於染料廢水以單一純脫色菌相培養進行生物處理，並不具高脫色效能，此研究即嘗試「人工建構」出對目標染料「反應紅22號」具高脫色效率之混合菌相。先前研究曾不預期而發現DH5α與Pseudomonas luteola之共存可顯著加強生物脫色之能力。雖然「非脫色菌」（大腸桿菌DH5α）在此混合菌群中表現出「休眠態」之代謝活性特質，但是其存在確實對脫色菌具明顯輔助「催化」之效果。此研究之重要性即是強調DH5α於生物脫色上仍具「活性加強」之特性DH5α之「利他」行為表現較不具此交互作用之單一P. luteola培養更能特別提高其脫色效果。pH、溫度、溶氧及染料濃度對脫色之影響更顯示出DH5α存在之貢獻。「連續批式培養」之結果亦指出P. luteola經連續馴養後，可因長期適應於含染料環境下而提高脫色活性，此點亦支持於「系列批式培養」之脫色效果會隨系列培養次數增加之結果。「非脫色菌」DH5α釋出之胞外代謝物顯著地加強P. luteola之脫色效率。

並列摘要

This study provides an attempt to construct a mixed consortium in high decolourization efficiency to a model dye- reactive red 22, since pure cultures of decolourizer have less colour removal capability in dye-bearing wastewater treatment. In the preceding study, we unintentionally found coexistence of DH5α and Pseudomonas luteola resulted in an enhancement for decolourization. Although non-decolourizers (Escherichia coli DH5α) were considered metabolically ”dormant” in the constructed mixed consortium, their presence still significantly enhanced decolourization performance of the decolourizers (P. luteola). The crucial finding of this study indicates that DH5α serves as an active microorganism for biodecolourization enhancement. This ”altruistic” characteristics of DH5α in mixed consortia gives rise to higher decolourization performance than that without such beneficial Interaction (i.e., pure P. luteola cultures). To indicate the contributions due to the presence of DH5α, decolourization performances at various pHs, temperature, dissolved oxygen, and dye concentrations were evaluated. Results of repeated batch cultures also showed that consecutive acclimation of P. luteola significantly increased decolourization capability due to longer ”age” in dye-containing environments for higher azo-reductase activity. This also supported progressive increases in decolourization performance in sequencing batch cultures. Extracellular metabolites released by non-decolourizers DH5α considerably enhanced the decolourization of P. luteola.

並列關鍵字

Pseudomonas luteola ； Dye-containing wastewater ； Mixed consortium ； Bacterial decolourization ； Constructed community