Patellofemoral pain syndrome is one of the common sports injuries at knee in female athletes. Many athletes have recently used the kinesio taping to relieve patellofemoral pain. The purpose of this study was to investigate the effect of kinesio taping on patellofemoral pain syndrome. Nine subjects were recruited and the differences of the knee kinematics were compared before and after the use of kinesio taping. The results showed that the maximum knee flexion significantly decreased after kinesio taping during stair ascend. The findings suggested that patellofemoral joint pressure could be reduced due to decreased knee flexion following kinesio taping. Kinesio taping would be beneficial to prevent articular surface wear and pain for the victims of patellofemoral pain syndrome.