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長葉紫菜(Porphyra dentata Kjellman)之培養-絲狀體越夏培養及殼孢子放出之條件

Culture Studies on the Porphyra dentata Kjellman-Conchocelis Cultivation and Conditions for Conchospore Liberation

摘要


本實驗嘗試縮短紫菜絲狀體在貝殼內的培養時間,除了在正常的時間(三月)使果孢子着生於貝殼外,分別在五、六、七月份以游離狀培養的絲狀體着生於貝殼,由於五月份開始氣溫增高,使絲状體的生長受阻。到十月底時的結果顯示,只有三月份着生的貝殼有殼孢子放出,其他月份的則無。不過在二月份着生的貝殼如給以適當的環境處理,五月份卽可有殼孢子放出。 在尋求殼孢子放出的條件方面,給以十八種不同光度、光週期和溫度的混合條件下,我們發現較低的光線(1500lux, 500lux)和較短的光照週期(8:(平均值)16,5:(平均值)19)有較多的殼孢子放出,在所試驗的兩個溫度(20℃,16℃)間,並沒有太大的差異。在所有的處理條件下,處理的第二天有大量殼孢子放出,以後陸續有孢子放出,20℃條件下第五天仍有一放出的高峯,而16℃時却無。

關鍵字

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並列摘要


The cultivation period for Porphyra dentate conchocelis on shells can be shortened by seeding free conchocelis fragments on the shells. But the initial growth period for conchocelis on the shells should be started at temperatures between 18-22℃ in order to that the filaments can spread well over the shell surface. If they are started at temperatures higher than 25℃, the conchocelis will not grow well on the shells and ultimately no effective conchosporangia will form. At 26℃, the best conditions for conchosporangia formation were found to be 500lux, 8:(average)16; 1500lux, 8:(average)16;and 3000lux, 8:(average)16. Using 18 different combinations of temperature, photoperiod and light intensity, the liberation of conchospores from shells was observed to be much more abundant at conditions of darker light intensities and shorter photoperiods than under conditions of brighter light intensities and longer photoperiods. The increase of light intensity had no effect on the stimulation of conchospore liberation. At 16℃ and 20℃, the most abundant liberation of conchospores occured on the second day after treatment. There was a second peak of conchospore liberation on the fifth day at 20℃, but none at 16℃.

並列關鍵字

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