利用台灣金線連無菌播種根莖誘導多倍體研究

In Vitro Induction of Polyploidy from Rhizomes of Anoectochilus formosanus

夏奇鈮(Chi-Ni Hsia) ； 黃健覃(Jian-Tan Huang) ； 陳威臣(Uei-Chern Chen) ； 曹進義(Chin-Yi Tsao) ； 梁淑惠(Shu-Hui Liang) ； 蔡新聲(Shin-Sheng Tsay)

《台灣農業研究》 58卷4期 (2009/12) Pp. 302-309

https://doi.org/10.6156/JTAR/2009.05804.06

摘要

本研究以台灣金線連(Anoectochilus formosanus Hayata)無菌播種不同週齡之根莖(rhizome)作為培植體，並以流式細胞儀檢測新生芽體之倍體數，探討秋水仙素濃度、處理時間及固、液體處理方式對多倍體誘導之影響。比較固態或液態培養基添加0、0.025、0.125及0.625mM秋水仙素培養2週，對12週齡根莖培植體誘導多倍體之效果，結果顯示以0.625mM秋水仙素液態培養者，多倍體誘導率為44.8%最佳。以10、11及14週齡之根莖培養於含0、0.025、0.25及2.5mM秋水仙素之固態培養基中3天，其中以14週齡根莖培植體在2.5mM秋水仙素培養下，多倍體誘導率達42.4%最佳，且因其新芽成活率高，獲得之多倍體植株數亦最高。以10週齡根莖於含有2.5mM秋水仙素之液體培養基中培養16、24、48及72小時，結果顯示培養16小時即可誘得多倍體，但以培養24小時之4倍體誘導率45.5%最高。綜合本研究之結果，顯示利用金線連無菌播種長出之根莖作為培植體，具有數量眾多且成活率高的優點，選擇適當週齡之根莖進行秋水仙素處理，例如以10週齡根莖在含有2.5mM秋水仙素之液體培養基中培養24小時，各種多倍體誘導率最高可達54.6%。

關鍵字

金線連 ； 微體培養 ； 秋水仙素 ； 倍數體 ； 流式細胞儀

並列摘要

This study was conducted to investigate effects of concentration, exposure duration of colchicine and treating colchicine with liquid or solid agar medium on in vitro induction of polyploidy from rhizomes of Anoectochilus formosanus. Ploidy of shoots derived from rhizome explants were analyzed using a flow cytometry. When the 12-week-old rhizome explants were cultured for two weeks in a solid agar medium or in a liquid medium containing 0, 0.025, 0.125, and 0.625 mM colchicine, the induction rate of polyploidy was the highest (44.8%) for the regenerated shoots in the liquid culture containing 0.625mM colchicine. When the 10-, 11-, and 14-week-old rhizome explants were cultured for 3 days on a solid agar medium containing 0, 0.025, 0.25, and 2.5 mM colchicine, the induction rate of polyploidy (42.4%) was the highest in the samples of 10-week-old and 14-week-old rhizomes on the medium containing 2.5mM colchicine. Moreover, the 14-week-old rhizomes produced higher number of polyploidy plants than that of 10-week-old rhizomes because the former had a higher survival rate. When the 10-week-old rhizomes were cultured for 16, 24, 48, and 72 hours in a liquid medium containing 2.5 mM colchicine, the earliest polyploidy shoots showed in the treatment of 16 hour but the highest rate of tetraploid was found in the treatment of 24 hour (45.5%). This study reveals that the 10-week-old rhizomes of A. formosanus are suitable explants for in vitro production of polyploidy plants as they produced high rate (54.6%) of polyploidy plants after treating for 24 hour in the liquid culture containing 2.5 mM colchicines.

並列關鍵字

Anoectochilus formosanus ； Micropropagation ； Colchicine ； Polyploidy ； Flow cytometry