Determination of Iodine in Human Sweat by Spectrophotometry

人體汗中碘分光光度儀分析法研究

毛義方(I-Fang Mao) ； 陳美蓮(Mei-Lien Chen) ； 林宜長(Yi-Chang Lin) ； 藍忠孚(Chung-Fu Lan)

《中華公共衛生雜誌》 15卷1期 (1996/02) Pp. 40-46

https://doi.org/10.6288/CJPH1996-15-01-03

摘要

本研究是建立使用分光光度儀測量人體汗液中碘含量之方法。將汗液樣本使用超音波振碎機使汗液均質化，然後使用強酸加入汗液中，使各類碘化合物轉化成碘酸物(iodate)，然後以此碘酸物催化As及Ce之氧化還原反應發生之呈色作為汗液中碘含量之測定依據。
結果顯示，本研究發展之方法對汗液中含碘量可作適當之定量，其準確度介於105.6~110.2%，其精確度CV值為3.4~5.7%，測定範圍為2~10ng/mL，利用本法取10名自願者之汗液樣本共40個，測得其汗液中含碘量，平均值為36.6±6.7μg/L。此研究提供了測量體液中含碘量的一個簡單、精確及可行之測定方法，可應用於流行病學及生理學上之研究。

關鍵字

碘濃度測定 ； 人體汗液

並列摘要

A spectrophotometric method for the determination of trace quantities of iodine in human sweat has been developed. The samples were completely homogenized by ultrasonication. Various forms of iodine in sweat were converted into iodate by acid digestion. The converted iodate then acted as a catalyst for the reduction reaction of Ce (Ⅳ) in the presence of As (Ⅲ) to develop color change for colorimetric analysis.
The accuracy and precision of this method were tested with the spike method. From the experimental results, this method has been proved to be accurate enough for the determination of total iodine concentration in human sweat samples ranging from 2 to 10 ng/mL. A total of 40 sweat samples of 10 volunteers were examined, and the average concentration of iodine was found to be 36.6±6.7 μg/L. These results suggest that this method is a simple, reliable, feasible and sensitive method for the determination of iodine concentration in human body fluids.

並列關鍵字

total iodine determination ； sweat