人體尿液中被發現核基質蛋白22(NMP-22)、3-羥基鄰氨基苯甲酸(3-HAA)及犬尿酸(KYNA)為檢測膀胱癌之指標分子。本研究中以水熱法於金電極表面成長奈米氧化鋅柱,其直徑約為265.31 ± 39.91 nm,並以溶劑揮發法製備出NMP-22、3-HAA及KYNA分子拓印聚乙烯乙烯醇於氧化鋅電極,以可攜式恆電位儀進行非侵入式的電化學檢測。NMP-22對於乙烯比例為27 mole%的聚乙烯乙烯醇有較佳的拓印效率值。以NMP-22拓印氧化鋅電極對比NMP-22拓印電極,在吸附濃度100 pg/mL時,拓印氧化鋅電極之電流差值為64.56 ± 3.46 uA/cm2,而拓印薄膜電極之電流差值為37.13 ± 2.16 uA/cm2,差異可達1.74倍;以3-HAA對拓印氧化鋅電極對比3-HAA拓印電極,在吸附濃度10 pg/mL時,拓印氧化鋅電極之電流差值為34.98 ± 0.63 uA/cm2,而拓印薄膜電極之電流差值為23.21 ± 1.00 uA/cm2,差異可達1.51倍;以KYNA對拓印氧化鋅電極對比KYNA拓印電極,在吸附濃度100 pg/mL時,拓印氧化鋅電極之電流差值為29.66 ± 1.24 uA/cm2,而拓印薄膜電極之電流差值為18.82 ± 2.02 uA/cm2,差異可高達1.58倍,證明成長氧化鋅確實能提升靈敏度。進行電化學干擾性測試,可得知三種分子拓印氧化鋅電極對於干擾因子影響程度低。以循環伏安法進行添加法量測檢體尿液,結果得知準確率最高可達95.53 ± 3.35 %。 本研究與成大醫院泌尿科醫生合作並進行人體臨床試驗,利用NMP22、3-HAA拓印奈米氧化鋅電極結合注射式微流體系統進行尿液檢體量測,其檢測膀胱癌之準確率分別為75.0%和87.5%。
Nuclear matrix protein no.22(NMP22), 3-Hydroxyanthranilic acid(3-HAA) and kynurenic acid(KYNA) were found in patients’ urine of with bladder cancer. In this work, Zinc oxide(ZnO) rods were hydrothermally grown on a gold substrate; the rods had an average length and diameter of 1.08±0.11 μm and 265.31±39.91 nm, respectively. NMP-22, 3-HAA and KYNA were imprinted into poly(ethylene-co-vinyl alcohol), EVAL, which were coated onto ZnO rod arrays. Using non-invasive electrochemical detection in urine samples by portable potentiostat. The higher imprinting effectiveness for NMP-22 was obtained with 27 mole % of ethylene. The electrochemical response with MIPs/ZnO electrodes was 1.5-fold higher than that with MIPs by the injection microfluidic CV system. In human clinical trials, the sensitivity of NMP-22 and 3-HAA were 75.0 and 87.5%.