Based on the different species of the cotton or the cotton's country of origin, the quality will be different; especially on the cotton's luster, even on the same plant the color might be different too. Therefore, many spinning mills adopted the traditional way in blended multiple cottons with a small amount of new cotton to avoid the color difference among the lots of grey yarn. However, the color difference sometimes is still obvious. This research will use the ICS spectra-colorimeter to build up the single cotton's yellow index and color difference, and then weighted estimate the luster of blended multiple cottons. The result found the estimated luster is very close to the measured value, which means this method is available.