Ultrasound is frequently applied for the evaluation of the diseased knee joint with effusion. Aspiration with injection of selected drugs were undertaken when necessary. The aspiration of synovial fluid from joints and inflamed tendons or bursae fulfills an important role in the diagnosis of rheumatic disease. Removing the excess fluid can provide relief of symptoms and preserve the joint integrity. Intra-articular injections are import as therapeutic modalities employed to threat a wide range of musculoskeletal disease. The knee joint is the easiest joint to be aspirated and also the most common joint for aspiration. Common reasons for aspirating or injecting a knee include local anesthesia, diagnosis of an unexplained effusion, drain off infected fluid, or instill medication. In this article, we discuss the facts about knee aspiration and injection, including indications, contraindications and complications.