臺灣產蟲生真菌Purpureocillium takamizusanense感染荔枝椿象（Tessaratoma papillosa）初報

First report of Purpureocillium takamizusanense as an omopathogenic fungus infecting Tessaratoma papillosa (Drury) in Taiwan

羅佩昕(Lo, P. H.) ； 于逸知(Yu, Y. C.) ； 白桂芳(Pai, K. F.)

《植物醫學》 61卷2&3期 (2019/09) Pp. 27-30

https://doi.org/10.6716/JPM.201909_61(2_3).0004

摘要

本研究自臺中與南投地區龍眼樹上採集疑似受蟲生真菌感染之荔枝椿象（Tessaratoma papillosa Drury），其氣孔與體節間隙可見到菌絲團並佈滿粉紫色之分生孢子。該菌體經分離純化後，依其形態與分子生物學特性加以鑑定，配合內轉錄間隔區（internal transcribed spacer）、增幅延長因子（translational elongation factor 1α）及β微管蛋白（β-tubulin）的基因序列片段，將其鑑定為Purpureocillium takamizusanense（Kobayasi）S.Ban, Azuma & Hirok. Sato。病原性測試結果，其分生孢子具感染荔枝椿象成蟲的能力，並於接種後14天開始造成蟲體死亡，至第28天致死率達85.2%，顯示該菌對防治荔枝椿象具有高度應用潛力。

關鍵字

荔枝椿象 ； 蟲生真菌 ； Purpureocillium takamizusanense

並列摘要

In this study, entomopathogenic fungi were isolated from dead Lychee stink bug (Tessaratoma papillosa) in Taichung and Nantou of Taiwan. White mats with pinkish purple conidia emerged from the intersegmental membranes of antennae, legs and body, and the spiracles of dead bugs. The fungi were identified as Purpureocillium takamizusanense (Kobayasi) S. Ban, Azuma & Hirok. Sato based on morphology and molecular characters, sequencing intergenic transcribed spacer (ITS) rDNA, transcribed elongation factor 1α (EF-α), and β-tubulin genes. Moreover, pathogenicity test showed that it killed adult bugs 14 days post-inoculation and had 85.2% mortality rate after 28 days of inoculation. Therefore, it shows high potential on biological control of Lychee stink bug in Taiwan.

並列關鍵字

Tessaratoma papillosa (Drury) ； entompathogenic fungi ； Purpureocillium takamizusanense