台灣禽類肉毒桿菌神經毒素之特性分析

Characterization of the Neurotoxin Produced from Avian Botulism in Taiwan

陳燕萍(Yen-Ping Chen) ； 郭恕君(Shu-Jyun Kuo) ； 鄭明珠(Ming-Chu Cheng) ； 李敏旭(Min-Shiuh Lee) ； 李淑慧(Shu-Hwae Lee) ； 蔡向榮(Hsiang-Jung Tsai)

《臺灣獸醫學雜誌》 34卷2期 (2008/06) Pp. 90-96

https://doi.org/10.7009/TVJ.200806.0090

摘要

於2002年12月至2006年11月共有15個疑似肉毒中毒之禽類病例，其中6個病例之檢體可偵測到肉毒毒素或其毒素基因，並已分離出2株肉毒桿菌。病例中禽鳥肉毒中毒病例相關之毒素可同時被C型與D型肉毒抗毒素血清所中和，利用肉毒桿菌C型與D型專一性引子進行PCR檢測，顯示這些肉毒神經毒素基因為由C型與D型神經毒素所組成，進一步將毒素基因經核酸序列分析，顯示這些毒素基因包括2/3之C型神經毒素基因與1/3之D型神經毒素基因。以上結果顯示，結合C型與D型之肉毒神經毒素可能為造成台灣禽類肉毒中毒的病因，此為台灣第一篇完整之報告。

關鍵字

肉毒桿菌 ； 肉毒中毒 ； 神經毒素 ； 毒素基因

並列摘要

There were 15 cases of suspected avian botulism between December 2002 and November 2006. Botulinum neurotoxin genes or neurotoxin could be detected in the specimens from 6 cases of avian botulism and two strains of Clostridium botulinum also have been isolated. We conducted neutralization tests of samples from cases of avian botulism, and the toxins related to avian botulism were neutralized with both type C and D antitoxins. The results of PCR with type C and D neurotoxin specific primers and an analysis of nucleotide sequences revealed that the neurotoxic gene from avian botulism comprised two thirds of the type C neurotoxin gene and one third of the type D neurotoxin gene. This indicated that the neurotoxin of avian botulism was a mosaic of type C and D neurotoxins and that the mosaic neurotoxin was probably a main etiology causing some forms of avian botulism in Taiwan. This was the first complete report in Taiwan.

並列關鍵字

Clostridium botulinum ； botulism ； neurotoxin ； neurotoxin gene