脆鐵線蕨孢子無菌撒播繁殖體系之建立

Partial Tissue Culture System for the Mass Propagation of Adiantum tenerum 'Scutum Roseum' from Spores

高秀雲(Hsiu-Yun Kao) ； 葉德銘(Der-Ming Yeh)

《台灣林業科學》 18卷1期 (2003/03) Pp. 33-42

https://doi.org/10.7075/TJFS.200303.0033

摘要

脆鐵線蕨(Adiantum tenerum 'Scutum Roseum')孢子播於無土介質及試管內，比較其原葉體生長及發育。孢子播於泥炭苔、蛭石與珍珠砂之無土混合介質下，原葉體長度約1.2mm、寬度約1.3mm以上時，即有藏精器的分化；原葉體長度約2.0mm、寬度約2.5mm，方觀察到藏卵器之形成。將孢子播於試管內，經增殖而來的原葉體僅分裂數個細胞後即有藏精器的形成。且於無菌撒播20d後，自翼片下側近假根處，開始有原葉體的增殖與鮮重之增加。於60d後，原葉體的鮮重即無明顯增加，而葉綠素含量下降。適當的繼代時期為無菌撒播後50-60d，於第一次繼代6-8wk後需再行第二次繼代，以使原葉體大量增生。若將無菌撒播60d之原葉體團，以全量強生氏營養液浸泡5sec後，再置於無土介質中，其孢子體形成速率，較前人所推薦之二分之一MS為佳。本研究建立以部分組織培養方式，可兼顧原葉體培育、增殖並可獲得更多的孢子體。

關鍵字

脆鐵線蕨 ； 孢子 ； 原葉體 ； 無菌繁殖

並列摘要

Comparative growth and development of gametophyes and sporophytes were determined in Adiantum tenerum 'Scutum Roseum' sown in a soilless mix and cultured in vitro. Prothallia in the soilless mix were competent to initiate antheridia provided that these prothallia had reached a critical size at a length of ＞1.2mm and a width of ＞1.3mm, while archegonia were observed with prothallia at a length of ＞2.0mm and a width of ＞2.5mm. In contrast, antheridia were observed in young adventitious prothallia cultured in vitro to have only a few cells. Young adventitious prothallia were differentiated from the lower region of wing cells of the prothallia after spores had been sown in vitro for20 d. After sowing for 60 d, the fresh weight of prothallia had increased due to adventitious outgrowths. The fresh weight of prothallia had increased slightly, and the chlorophyll content had begun to decrease after having been sown in vitro for 60 d. The first optimum in vitro transfer duration was approximately 50-60 d after sowing, and the second subculture was 6-8wk after the former transfer. Prothallia soaked with Johnson's solution for 5 sec before transfer from in vitro culture to the soilless mix produced faster sporophyte formation than those soaked with 1/2 MS as previously recommended. The established partial tissue culture system could facilitate both the mass propagation of prothallia and production of a greater number of sporophytes.

並列關鍵字

Adiantum tenerum 'Scutum Roseum' ； spore ； prothallium ； in vitro propagation