牛樟芝培養與分析研究探討

Studies on cultivation and compounds analysis of Antrodia camphorata

凌士廷(Shih-Ting Ling)

指導教授 ： 曾耀銘

朝陽科技大學/理工學院/生化科技研究所/碩士(2010年)

摘要

樟芝(Antrodia camphorata)，又名牛樟芝及牛樟芝，是屬於擔子菌門(Basidiomycota)。樟芝在台灣通常用來預防及治療各種疾病，包括酒精中毒、毒品中毒、皮膚癌及各種癌症。僅生長在台灣特有牛樟樹上(Cinnamomum kanehirai Hay)。由許多分離與分析研究得知，除了多醣體(Polysaccharides)之外，樟芝有許多的生理活性成分化合物，包括萜類(terpenoids)、苯類(benzenoids)、木脂素(lignans)、苯的衍生物(benzoquinone derivatives)、琥珀和馬來酸衍生物(succinic and maleic derivatives)。在此研究發現培養時的溫度、相對溼度及光線對於樟芝椴木培養的生長影響甚大。更發現椴木培養之菌絲體的菌絲厚度，在菌種型態上的轉化有極大的關鍵影響。

另一方面，此研究結果說明，椴木培養之菌絲體與子實體在三萜類化合物的產量上有明顯的變化。以不同生長時期的一次菌絲體和二次菌絲體於HPLC測定內含指標性三萜類化合物含量。此外，本實驗室椴木培養之樣品與市售商品於HPLC進行三萜類化合物含量比較。

關鍵字

HPLC定量分析 ； 牛樟芝 ； 椴木培養條件 ； 菌絲體/子實體 ； 三萜類化合物

並列摘要

Antrodia camphorata (Niu-Chang-Chih or Niu-Chan-Ku), Polyporaceae, is a medicinal mushroom that has been used for centuries in Taiwan for the prevention and treatment of various diseases including alcohol and drug intoxication, skin itching and, cancer. A. camphorata is grown on the hardwood of endemic species Cinnamomum kanehirai Hay (Lauraceae). A. camphorata is consisting of various bioactive compounds include terpenoids, benzenoids, lignans, benzoquinone derivatives, succinic and maleic derivatives, in addition to polysaccharides. In this study, we found that that the cultivation temperature, relative humidity and the light have significant effect on the growth of A. camphorata in the wood cultivation method. We also found that the wood cultivation period has noticeable effect on the thickness of the grown mycelium and its morphology.

On the other hand, our results also indicated that there is a significant variation in the yield of triterpenoids from the wood grown mycelium as well as fruiting bodies. We have quantitatively determined the major triterpenoids using HPLC in the wood cultivation grown primary and secondary mycelium of A. camphorata at different growth periods. Furthermore, the HPLC triterpenoids quantitative data from our wood cultivation grown A. camphorata were compared with commercial samples.

並列關鍵字

quantitative determination using HPLC ； Antrodia camphorata ； Triterpenoids ； mycelium/fruiting bodies ； Wood cultivation condition

參考文獻

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