We present a flow chart for the determination of eleven intermediates of chlorophyll biosynthesis and degradation pathways. This flow chart is organized from six literatures. We just add a step to link two unrelated procedures to determine the content of pheophytin a and b. It is convenient for the people who want to determine the content of chlorophylls and their derivatives. This flow chart makes it possible to determine the content of eleven pigments of more than 100 samples in one work day.