Genetic variation and population structure of 113 individuals sampled from 13 provenances of Casuarina junghuhniana Miq. were examined using inter simple sequence repeat (ISSR) fingerprinting. The 8 ISSR primers assayed yielded 52 polymorphic bands. Analysis of molecular variance (AMOVA) revealed that the variance component between native and cultivated provenance groups was 14.31% (p<0.01), among provenances within groups was 10.33% (p<0.001), and among individuals within provenances was 75.36% (p<0.001). Remarkable genetic differentiation and low levels of gene flow were detected between native provenances due to geographical isolation. In contrast, cultivated provenances had low genetic differentiation perhaps due to coancestry. The UPGMA analysis revealed that the cultivated provenances from Kenya and Tanzania might have originated from native provenances from East Java, Lombok, Bali, and Sumbawa. The native provenances from Timor revealed geographic relationships among these islands.
Genetic variation and population structure of 113 individuals sampled from 13 provenances of Casuarina junghuhniana Miq. were examined using inter simple sequence repeat (ISSR) fingerprinting. The 8 ISSR primers assayed yielded 52 polymorphic bands. Analysis of molecular variance (AMOVA) revealed that the variance component between native and cultivated provenance groups was 14.31% (p<0.01), among provenances within groups was 10.33% (p<0.001), and among individuals within provenances was 75.36% (p<0.001). Remarkable genetic differentiation and low levels of gene flow were detected between native provenances due to geographical isolation. In contrast, cultivated provenances had low genetic differentiation perhaps due to coancestry. The UPGMA analysis revealed that the cultivated provenances from Kenya and Tanzania might have originated from native provenances from East Java, Lombok, Bali, and Sumbawa. The native provenances from Timor revealed geographic relationships among these islands.