Abstract Tyramine exists abundantly in dairy products such as cheese and yogurt; which is an indirectly acting sympathomimetic amine. Tyramine is largely inactivated by metabolism to catalyzed by monoamine oxidase (MAO) enzymes located in the liver, gut, and sympathetic nerves. The pressor action of tyramine was found to be dramatically potentiated in patients who take cheese while receiving MAO inhibitors (MAOI). This “cheese-reaction” restricts the drug use in the treatment of depression. High blood tyramine concentration may lead to high blood pressure due to the increase of norepinephrine. This phenomenon is commonly seen in people with depression or administrated with monoamine oxidase inhibitor. Therefore the measurement of serum tyramine concentration becomes critical. In this study, we detected serum tyramine concentration by molecular imprinted polymers (MIP) and modified screen printed carbon electrodes (SPCE). The electrodes were modified with PEDOT/PSS and gold nanoparticles (PEDOT/PSS/AuNP) to enhance the conductivity. The electrode was further modified with positively charged 1-methyl-4-mercaptopyridine (1-m-4-MP) byAu-S bonding to electrostaticly attract negatively charged tyramine at basic condition. Differential pulse voltammetry (DPV) was employed for the detection. The limit of detection (LOD), limit of quantitation (LOQ) and linear range of tyramine was 1.17 nM, 3.94 nM and 1×10-7∼5×10-9 M, respectively.