植酸酵素基因轉殖馬鈴薯收穫田區之殘株管理－浸水處理評估

Effect of Flooding on Management of Phytase-Containing Potato Tubers in the Harvested Field

陳烈夫(Lit-Fu Chen) ； 吳明哲(Min-Tze Wu) ； 呂秀英(Hsiu-Ying Lu)

《台灣農業研究》 57卷2期 (2008/06) Pp. 95-102

https://doi.org/10.6156/JTAR/2008.05702.02

摘要

馬鈴薯(Solanum tuberosum L.)是世界上最重要的非禾穀類糧食作物。基因轉殖馬鈴薯2-1品系，是由‘克尼伯’(Kennebec)品種轉殖植酸酵素基因(phytase gene)所產生的基因轉殖作物。爲了探討浸水處理對基因轉殖馬鈴薯收穫田區之殘株管理的效果，本研究在農業試驗所於2006年秋作種植的收穫田區內進行相關試驗。試驗田間設計採兩因子之裂區設計，三區集，以浸水或不浸水兩種處理爲主區，轉殖和非轉殖兩品（種）系爲副區。浸水區在試驗期間一直保持水深淹過田面3 cm。浸水後第7、17、28、51、70天分別自每小區內各逢機取出4株，調查取樣植株上的殘留薯球總數，以進行差異性比較。結果發現，浸水17天起處理之間的殘留薯球數量即有顯著差異，浸水28天以上，轉殖與非轉殖馬鈴薯殘株皆可被完全清除。本試驗評估結果顯示浸水爲清除轉殖馬鈴薯土壤中殘薯的有效管理方法；此外，‘克尼伯’轉殖2-1品系的田間棄置殘株產量明顯低於‘克尼伯’，亦可供生物安全評估之參考。

關鍵字

馬鈴薯 ； 基因轉殖作物 ； 植酸酵素基因 ； 殘株管理 ； 浸水

並列摘要

Potato (Solanum tuberosum L.) is the most important non-cereal food crop in the world. The transgenic potato plants, strain 2-1, were obtained by transforming phytase-gene into their original plants, cv. 'Kennebec' (wild type). To study effects of flooding on survival and persistence of transgenic potato tubers in the harvested field, an experiment was conducted in a field at the Agricultural Research Institute in the fall crop season of 2006. A factorial split design with three blocks was used with flooding (with and without) as main factor and cultivars (strain 2-1 and wild type) as subfactors. For the flooding treatment, water level was maintained at 3 cm above soil, starting at harvest time and throughout the entire period of the experiment. For each of sampling date at 7, 17, 28, 51, and 70 days after flooding, four potato plants were randomly sampled from each plot and examined for number of tubers. Results showed that number of tubers was significantly reduced after flooding treatment of filed plots for 17 days, compared to non-flooding controls. After flooding for 28 days or longer, no tubers were found on both transgenic and non-transgenic plants of potatoes. This results suggest that flooding of potato fields is an effective method for post harvest field management of transgenic potatoes. Besides, production of potato tubers in the transgenic plants 'Kennebec' strain 2-1 was significantly lower than the non-transgenic plants cv. 'Kennebec' sampled from plots without flooding treatments. The lower number of potato tubers in the transgenic plants 'Kennebec' strain 2-1 might be another reference point for consideration in biosafety assessment of growing transgenic potatoes in the field.

並列關鍵字

Potato ； Solanum tuberosum ； Genetically modified crops ； Phytase gene ； Residue management ； Flooding