培植體大小、蔗糖及活性炭對孤挺花組培苗增殖與生長之影響

Effect of Explant Size, Sucrose and Activated Charcoal on In Vitro Plantlet Proliferation and Growth in Amaryllis

陳威臣(Uei-Chern Chen) ； 曹進義(Jhin-Yi Tsao) ； 吳姿穎(Tzu-Ying Wu) ； 夏奇鈮(Chi-Ni Hsia)

《台灣農業研究》 68卷2期 (2019/06) Pp. 137-147

https://doi.org/10.6156/JTAR.201906_68(2).0004

摘要

本研究以孤挺花「台農1號」（Hippeastrum hybridum Hort. 'Tainung No.1'）組培苗為材料，探討培植體大小、蔗糖及活性炭濃度對組培苗生長之影響。以鱗莖直徑約3、6、9mm之4分縱切鱗莖作為培植體進行培養，結果顯示不同大小培植體之組培苗增殖率並無顯著差異。每個1/4鱗莖於培養8 wk後，約可形成1.1－1.3株組培苗。此外，9 mm處理之培植體，在8 wk培養後可獲得具有較大鱗莖之組培苗；然而，在延長培養時間至12 wk後，9 mm與6 mm處理間已無顯著差異。利用鱗莖直徑約3－5mm之組培苗作為培植體，進行1.5－9.0%蔗糖與0－2g L^(-1)活性炭濃度組合之複因子試驗，結果顯示6.0%蔗糖配合1.0－2.0 gL^(-1)活性炭處理，對於增加組培苗鱗莖直徑與鮮重較為有利，分別達到8 mm與1.73－1.75 g；但是當蔗糖濃度達到9%時，則會抑制組培苗之生長。就組培苗根數而言，在含有6%蔗糖搭配0.5－2.0 gL^(-1)活性炭條件下，可形成8.8－9.6條根，均較高於其他處理。就組培苗葉數而言，於0.5－2.0 gL^(-1)活性炭濃度下，蔗糖濃度在1.5－6.0%之間並無顯著差異。綜合而言，蔗糖濃度自1.5%提高至6.0%，可增加組培苗之鱗莖直徑、鮮重及根數，若同時添加適量活性炭，則具有協同增效蔗糖之效果。

關鍵字

石蒜花 ； 四分切鱗莖 ； 大量繁殖 ； 發根 ； 增效作用

並列摘要

Factors of explant size, concentration of sucrose and activated charcoal in the culture medium were investigated on in vitro growth of Hippeastrum hybridum Hort. 'Tainung No. 1' plantlet in this study. One-quarter vertical cut bulb explants derived from 3, 6 and 9 mm diameters of bulb were evaluated after 8 wk and 12 wk of culture. Proliferation rate of 1.1-1.3 plantlets per one-quarter bulb explant was obtained after 8 wk of culture, and no significant difference was found among all treatments. Furthermore, plantlets with larger bulb were obtained from explants derived from explants of 9 mm treatment. However, no significant difference on bulb diameter of 6 mm and 9 mm treatments was found after extending culture time to 12 wk of culture. A factorial experiment using 1.5-9.0% sucrose in combination with 0-2.0 g L^(-1) activated charcoal was conducted using explants derived from plantlets with 3-5 mm diameter of bulb. The better results were obtained from 6% sucrose in combination with 1.0-2.0 g L^(-1) activated charcoal on increase of bulb diameter and fresh weight which were 8.0 mm bulb diameter and 1.73-1.75 g fresh weight per plantlet, respectively. It was also found that growth of plantlets was inhibited in the medium containing 9.0% sucrose. In terms of the root number of plantlet, the best results of 8.8-9.6 roots per plantlet were obtained from 6.0% sucrose in combination with 0.5-2.0 g L^(-1) activated charcoal, and higher than the other treatments. For the leaf number of plantlet, no significant difference was found by using 1.5-6.0% sucrose in combination with 0.5-2.0 g L^(-1) activated charcoal. In summary, raising sucrose concentration from 1.5% to 6.0% would increase bulb diameter, fresh weight and root number of plantlet, and adding proper concentration of activated charcoal would has a synergistic effect with sucrose.

並列關鍵字

Hippeastrum hybridum Hort. ； One-quarter bulb ； Masspropagation ； Rooting ； Synergistic effect