簡訊：台灣地區鴿子披衣菌（壁衣體）之分離與血清學調查

民國85年7月到86年4月間，自台灣地區9縣市的43戶養鴿舍，共計採得731隻鴿血清及668個泄殖腔拭子檢體。另於民國85年7月至8月，自台北市凡賽爾賽鴿醫院，提供10縣市28戶鴿舍送診糞便，計71件。採得之血清以德國製的RIDASCREEN（superscript R） Chlamydia psittaci競爭性酵素免疫分析法檢測試劑組檢測，結果鴿子抗體陽性率為43.4%（317/731），鴿舍抗體陽性率為97.7%（42/43）。泄殖腔拭子及糞便檢體，經接種SPF雞胚胎卵黃囊一代後，再於McCoy細胞繼代後以C. psittaci特異性單株抗體做間接螢光抗體法檢查，陽性檢體並以Giemsa及modified Macchiavello、Gimenez法染色及電子顯微鏡鏡檢，證明披表菌之原質小體（elementary body）存在。結果分離率為5.1%（38/739），其中在668隻泄殖腔拭子檢體及71件糞便中，分離率分別為5.4%（3 6/668）及2.8%（2/71）。本調查為台灣首次之鴿子披衣菌分離及血清學調查，由結果證實台灣地區鴿子披衣菌感染情形相當普遍，在公共衛生上值得重視。

關鍵字

鴿子；披衣菌；壁衣體；分離；血清學調查

並列摘要

From July 1996 to April 1997, a total of 731 serum samples and 668 cloacal swabs from 43 lofts in 9 prefectures were collected. Other 71 fecal specimens from 28 lofts in 10 prefectures received by Versele-Laga pigeon's hospital were also included. Serum samples were examined for antibodies against Chlamydia psittaci by a commercial RIDASCREENR EIA kit. The results indicated that antibody positive rate was 43.4% (3171731) in tested serum samples. The loft positive rate was 97.7% (42/ 43). Cloacal swabs and fecal specimens were inoculated into yolk sac of SPF chicken embryos, and then onto McCoy cells. The presence of chlamydia anti gen was confirmed by an ind irect immunofluorescence assay using monoclonal antibodies against C. psittaci. Elementary bodies in the infected cells were also demonstrated by the Giemsa, modified Macchiavello and Gimenez staining and by the electron microscopy. In 668 cloacal swabs and 71 fecal specimens, the isolation rate was 5.4% (36/668) and 2.8% (2/71), respectively. Total positive rate was 5.1% (38/739). This is the first report on C. psittaci isolat ion and antibody survey in pigeons in Taiwan.

並列關鍵字

Pigeons ； Chlamydia psittaci ； Isolation ； Antibody survey

國際替代計量

簡訊：台灣地區鴿子披衣菌（壁衣體）之分離與血清學調查

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