Measurement of Human Endothelial Cell Adhesion Using Dielectrophoresis

以介電泳量測人類內皮細胞之貼附力

洪敏勝(Min-Sheng Hung) ； 陳佑瑋(Yu-Wei Chen) ； 林照傑(Chao-Jie Lin) ； 艾群(Chyung Ay) ； 邱欽平(Chin-Ping Chiou)

《中國機械工程學刊》 30卷5期 (2009/10) Pp. 393-400

https://doi.org/10.29979/JCSME.200910.0004

摘要

本研究應用介電泳分別探討人類臍靜脈內皮細胞(ECV304)培養於玻璃以及聚二甲基矽氧烷時之貼附力。結果顯示，在37℃時細胞可以成功培養在塗佈有膠原蛋白之玻璃及聚二甲基矽氧烷的表面。由介電泳力之計算，細胞培養在玻璃上1小時，其貼附力為培養7小時時的32%。培養在PDMS上1小時，其貼附力為培養7小時時的21%。對於培養在二種材料上，細胞貼附力均隨著培養時間的增加而增加。而細胞在初期培養與6小時培養後其貼附力增加，與焦點黏附激酶的活化現象有關。此外，細胞培養於玻璃上比培養於PDMS上有較佳的貼附力。

並列摘要

This study measures adhesion force of human endothelial cell (ECV304) using dielectrophoresis. This study cultures ECV304 cells on polydimethylsiloxane (PDMS) surface and glass surfaces. Experimental results show that the ECV304 cells can attach and reproduce on the PDMS surface and the glass surface with a collagen coating (Rat Tail Tendon Type I) at 37℃. At a seeding time of 1 hour, the cell adhesion force on the glass surface and on the PDMS surfaces is about 32% and 21% of the cell adhesion force at a seeding time of 7 hours, respectively. The cell adhesion force increases as the seeding time increases, for both PDMS and glass surfaces. The cell adhesion strength increases at the early stages of adhesion and after the seeding time of 6 hours because of the focal adhesion kinase activation. In addition, cell seeded on the glass exhibited better cell adhesion than cells seeded on PDMS, perhaps due to partial denaturation or changes in integrin orientation on the hydrophobic surface.

並列關鍵字

cell adhesion force ； endothelial cell ； dielectrophoresis