Reducing energy use for space cooling in buildings is a key measure to energy conservation and environmental protection in places having hot and humid weather, such as Taiwan. In this research, we use two identical experimental houses with the climate monitoring system to estimate the energy consumption of air-conditioning system with different building envelope strategies (the U-value of the overall wall and roof, the external and internal shading device, the window opening area ratio, etc.). We also adopted the energy simulation free software eQUEST, which is based on the well-known DOE-2 program, to estimate the energy saving of different strategies. Actual building and operating data were fed to eQUEST to calculate the instantaneous electric consumption due to air conditioning and the comparison between measured and predicted electric consumption is presented to understand the accuracy of the energy simulation program.